HPLC测定龙血竭脂质纳米粒中龙血素A的含量

Determination of Loureirin A in Solid Lipid Nanoparticles Encapsulating Resina Draconis by HPLC

关键词:  高效液相色谱法  龙血竭  龙血素A  脂质纳米粒

Key words:  HPLC  Resina Draconis  loureirin A  solid lipid nanoparticle

摘要:

目的 采用HPLC测定龙血竭脂质纳米粒中龙血素A的含量并计算其包封率。方法 采用DiamonsilTM C18(200 mm×4.6 mm，5 μm)色谱柱，以乙腈-1%冰醋酸水溶液(38∶62)为流动相，流速为1.3 mL·min^-1，检测波长为260 nm，柱温为40 ℃，测定样品中龙血素A的含量并计算其包封率。结果 龙血素A 于0.135~5.4 μg·mL^-1内有良好线性关系，Y=33.694X+1.589 8(r=0.999 9)。低、中、高3个浓度的日内RSD分别为2.70%，2.36%，1.48%；日间RSD分别为2.70%，2.61%，1.97%，该方法的平均回收率为99.2%，RSD为3.9%，平均包封率为89.59%。结论 本方法准确、简便、重复性好，可用于龙血竭脂质纳米粒中龙血素A含量的测定。

Abstract:

OBJECTIVE To establish an HPLC method for determination of loureirin A content in solid lipid nanoparticles(SLN) of Resina Draconis. METHODS A DiamonsilTM C18(200 mm×4.6 mm, 5 μm) column was used for the determination of loureirin A with mobile phase of acetonitrile-1% acetic acid solution (38∶62). The flow rate was 1.3 mL·min^-1, the detection wavelength was set at 260 nm, and the column temperature was 40 ℃. RESULTS The linear range of 1oureirin A was 0.135-5.4 μg·mL^-1, Y=33.694X+1.589 8(r=0.999 9). The RSDs of within-day were 2.70%, 2.36%, 1.48%, and the RSDs of between-day were 2.70%, 2.61%, 1.97% in the high-, middle-, and low-dose Resina Draconis loaded SLN. The average recovery was 99.2%(RSD 3.9%), and the average drug encapsulation efficiency was 89.59%. CONCLUSION The method is simple and reliable for the determination of loureirin A in Resina Draconis loaded SLN.