HPLC测定热熔压敏胶贴剂中α-细辛醚的含量

Determination of α-Asarone in Hot Melt Pressure Sensitive Adhesive Patches by HPLC

关键词:  α-细辛醚  热熔压敏胶  贴剂  高效液相色谱法

Key words:  α-asarone  hot melt pressure sensitive adhesive  patch  HPLC

摘要:

目的 建立HPLC测定热熔压敏胶贴剂中α-细辛醚含量的方法。方法 以甲苯为萃取溶剂提取药物，采用ZORBAX Eclipse XDB-C₁₈色谱柱(150 mm×4.6 mm，5 μm)，以甲醇-水(55∶45)为流动相，流速：1.0 mL·min^-1，检测波长：311 nm，柱温：40 ℃。结果 α-细辛醚在8.032~18.07 μg·mL^-1内线性关系良好(r=0.999 9)，回收率为99.3%(RSD=0.99%)，供试品溶液在室温下48 h内稳定。结论 本方法操作简单，结果准确，可用于热熔压敏胶贴剂中α-细辛醚的含量测定。

Abstract:

OBJECTIVE To establish an HPLC method for determination of α-asarone in hot melt pressure sensitive adhesive patches. METHODS Toluene was used as the exacted solvent. The column was ZORBAX Eclipse XDB-C₁₈(150 mm × 4.6 mm, 5 μm). The mobile phase was methanol-water (55∶45) and the flow rate was 1 mL·min^-1. The detection wave length was 311 nm. The column temperature was kept at 40 ℃. RESULTS A good linearity was obtained in a range of 8.032-18.07 μg·mL^-1 (r=0.999 9), and the recovery was 99.3%(RSD=0.99%). The solution was stable within 48 h under room temperature. CONCLUSION The method is simple and accurate, and is suitable for the determination of α-asarone in hot melt pressure sensitive adhesive patches.