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引用本文:方洁,张剑萍,张建华.应用UPLC研究亚胺培南在大鼠体内的药动学[J].中国现代应用药学,2013,30(3):250-253.
FANG Jie,ZHANG Jianping,ZHANG Jianhua.A Simple UPLC Method for Determination of Imipenem in Rat Plasma and Its Application in Pharmacokinetic Study[J].Chin J Mod Appl Pharm(中国现代应用药学),2013,30(3):250-253.
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应用UPLC研究亚胺培南在大鼠体内的药动学
方洁1, 张剑萍2, 张建华1
1.上海交通大学附属第六人民医院,儿内科,上海 200233;2.上海交通大学附属第六人民医院,药剂科,上海 200233
摘要:
目的 建立灵敏的超高效液相色谱法测定大鼠血浆中亚胺培南的浓度。方法 血浆样品采用乙腈蛋白沉淀方法,色谱柱为Dikma Diamonsil C18;以0.1 mol·L-1磷酸氢二钠(85%磷酸调pH至7.0)-甲醇(45∶55)为流动相;流速为1.0 mL·minL-1;柱温为35 ℃;检测波长为295 nm。结果 亚胺培南血药浓度在0.5~100 μg·mL-1内线性关系良好(r=0.999 7),最低检测限为0.5 μg·mL-1;日内、日间RSD均≤10%,提取回收率在80.5%~81.2%之间。6只SD大鼠单剂量口服给予亚胺培南后药动学参数分别为:Cmax(75.3±6.2)μg·mL-1t1/2(6.72±1.58)h;AUC0-t(694.1±28.3)h·μg·mLL-1;AUC0-∞(746.2±32.9) h·μg·mL-1结论 本方法简便、准确、灵敏、专属性强,同样适用于人血浆中亚胺培南浓度的测定及其药动学研究,对于评价亚胺培南疗效和安全性有重要意义。
关键词:  亚胺培南  超高效液相色谱法  药动学
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A Simple UPLC Method for Determination of Imipenem in Rat Plasma and Its Application in Pharmacokinetic Study
FANG Jie1, ZHANG Jianping2, ZHANG Jianhua1
1.Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University, Department of Pediatrics, Shanghai 200233, China;2.Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University, Department of Pharmacy, Shanghai 200233, China
Abstract:
OBJECTIVE The purpose of this study was to develop a simple and sensitive UPLC method to quantify imipenem in plasma. METHODS The chromatographic separation was carried out on a Dikma Diamonsil C18 column with a mobile phase consisting of 0.1M Na2HPO4 (85%H3PO4 adjust to pH 7.0)-methonal (45∶55) at a flow rate of 1.0 mL·min-1. The temperature of column was 35 ℃. UV wavelength mode was used, with imipenem monitored at 295 nm. RESULTS The calibration was linear in the range of 0.5-100 μg·mL-1 for imipenem. Inter- and intra-day precision were less than 10% for imipenem, respectively. The recovery was 80.5%-81.2%. The pharmacokinetic parameters were: Cmax (75.3±6.2)μg·mL-1; t1/2 (6.72±1.58)h; AUC0-t (694.1±28.3)h·μg·mL-1; AUC0-∞(746.2±32.9)h·μg·mL-1. CONCLUSION This simple, sensitive, accurate and cost-effective method can be used in routine clinical practice to monitor imipenem concentrations in plasma for patients.
Key words:  imipenem  UPLC  pharmacokinetics
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