山楂叶总黄酮对家兔动脉粥样硬化作用的机理研究

Mechanisms of Total Flavones of Crataegus Leaves on Experimental Atherosclerosis in Rabbits

关键词:  动脉粥样硬化  山楂叶总黄酮  C反应蛋白  IL-6  TNF-α  COX-ⅡmRNA

Key words:  atherosclerosis  TFCL  CRP  IL-6  TNF-α  COX-ⅡmRNA

摘要:

目的 研究山楂叶总黄酮(total flavones of crataegus leaves，TFCL)对家兔动脉粥样硬化(AS)形成抑制作用的机理。方法 用复方高脂饲料复制家兔AS模型并同时给予TFCL 80 d，取主动脉进行病理切片和大体观察，常规检测血脂和血清中两种抗氧化酶SOD、GSH-PX的活性和NO水平，ELISA法测定C反应蛋白(CRP)、IL-6及TNF-α的量，RT-PCR法检测COX-ⅡmRNA表达。结果 复方高脂饲料饲喂家兔80 d可以形成明显的AS，TFCL在减轻AS的同时能够降低血清中NO、CRP、IL-6及TNF-α的水平，升高SOD和GSH-PX的活性，下调 COX-ⅡmRNA的表达。结论 TFCL对复方高脂饲料形成的家兔AS具有一定的拮抗作用，提高抗氧化酶的活性、抑制炎性因子的生成可能是TFCL抗家兔AS的作用机理。

Abstract:

OBJECTIVE To investigate the mechanisms of total flavones of crataegus leaves (TFCL) on experimental atherosclerosis(AS) in rabbits. METHODS The typical atherosclerosis in rabbits was formed by administrating with high-lipid food or together with TFCL for 80 d. The section of aortia were examined pathologically. The activities of SOD, GSH-PX, the level of NO, CRP, IL-6 and TNF-α in the serum and the relative amount of COX-ⅡmRNA in aortia of atherosclerotic rabbits were detected. RESULTS Atherosclerotic rabbit models were replicated by feeding high-lipid food. TFCL could inhibit the formation of AS with decreasing the level of NO, CRP, IL-6 and TNF-α, increasing the activities of SOD and GSH-PX, down regulating the expression of COX-ⅡmRNA. CONCLUSION The protective effect of TFCL on atherosclerosis may result from elevating the activities of oxygen enzymes but suppressing the production of inflammation factor.