香青兰总黄酮对哮喘大鼠气道炎症及高反应性的改善作用

Inhibitory Effects of moldavica total flavone on Airway Inflammation and Hyperresponsiveness in Ovalbumin-induced Asthmatic Rats

关键词:  香青兰总黄酮  哮喘  气道炎症  高反应性

Key words:  moldavica total flavone  asthma  inflammation  hyperresponsiveness

摘要:

目的 研究香青兰总黄酮对哮喘大鼠气道炎症及高反应性的影响。方法 采用氨水引咳实验和磷酸组胺-氯化乙酰胆碱引喘实验观察香青兰总黄酮的镇咳、平喘作用；同时观察香青兰总黄酮对哮喘大鼠支气管肺泡灌洗液(BALF)中白细胞分类计数和离体气管螺旋条张力的影响。结果 香青兰总黄酮中、高剂量能延长小鼠咳嗽潜伏期，减少小鼠2 min内咳嗽次数(P<0.05或P<0.01)，延长豚鼠引喘潜伏期(P<0.01)；降低哮喘大鼠血清卵白蛋白特异性IgE水平(P<0.01)，减少BALF中嗜酸性粒细胞数量(P<0.01)及减小离体气管螺旋条张力(P<0.05或P<0.01)。结论 香青兰总黄酮具有一定镇咳、平喘作用；可改善哮喘气道炎症及高反应性。

Abstract:

Objective To investigate the effects of moldavica total flavone on airway inflammation and hyperrespon- siveness in ovalbumin-induced asthmatic rats. Methods The cough model in rats and asthma model in cavies were made by using ammonia water and histamine-acetylcholine chloride to observe the antitussive and antiasthmatic actions. The inhibitory effect on airway inflammation and hyperresponsiveness in ovalbumin-induced asthmatic rats were observed. Resluts The moldavica total flavone in middle and high dose group could extend the cough latent period in rats(P<0.05 or 0.01) and gasp latent period in cavies (P<0.01), decreased the levels of serum ovalbumin specific IgE (P<0.01) and eosinophil counts in bronchoalveolar lavage fluid (P<0.01), attenuate the contraction of tracheal smooth muscle in ovalbumin-induced asthmatic rats (P<0.05 or 0.01). Conclusion　 Moldavica total flavone has antitussive and antiasthmatic actions and may inhibit ariway inflammation and hyperresponsiveness in asthma.