谷氨酰胺对COPD外周血NF-κB及HSP70表达的影响

Effects of Glutamine on Expression of Nuclear Factor-κB and Heat Shock Protein 70 in PBMC of Patients with COPD

关键词:  慢性阻塞性肺疾病  谷氨酰胺  核因子-κB  热休克蛋白70

Key words:  chronic obstructive pulmonary disease  glutamine  nuclear factor-κB  heat shock protein 70

摘要:

目的 探讨谷氨酰胺(Gln)对慢性阻塞性肺疾病(COPD)患者外周血单个核细胞(PBMC)中核因子-κB(NF-κB)和热休克蛋白70(HSP70)表达的影响。方法 选择30例COPD急性加重期(AECOPD)患者为研究对象，设为AECOPD组，将治疗10~20 d后处于稳定期的上述患者设为SCOPD组，分别以Gln干预，收集干预前后各组外周血PBMC，并以健康人为正常对照组。采用Real-time PCR法检测各组PBMC中NF-κB P65 mRNA 和HSP70 mRNA的表达水平。 结果 AECOPD和SCOPD组NF-κB P65、HSP70的表达高于正常对照组(P<0.05)，且急性期增高更为显著；AECOPD组和SCOPD组中，用Gln干预的较未用Gln的PBMC中HSP70表达升高(P<0.05)，而NF-κB P65表达下降(P<0.01)。结论 Gln可抑制COPD患者炎症因子NF-κB的活性，升高HSP70的表达。

Abstract:

OBJECTIVE To discuss the effects of glutamine on the nuclear factor factor-κB(NF-κB) and heat shock protein 70(HSP70) in peripheral blood mononuclear cells(PBMC) of patients with chronic obstructive pulmonary disease(COPD). METHODS Thirty patients with acute exacerbtion of COPD(AECOPD) were improved to stable stage(SCOPD), and then divided into two groups: blank control group and Gln group. Fifteen healthy people were in the normal group. The levels of NF-κB and HSP70 gene expression were detected by real-time PCR. RESULTS The levels of gene expression of NF-κB and HSP700 in blank control of AECOPD and SCOPD were higher than those in the normal group(P<0.05). After glutamine treatment, the expression of HSP70 increased in both AECOPD and SCOPD(P<0.05), while NF-κB P65 decreased(P<0.01). CONCLUSION Glutamine can inhibit expression of NF-κB and increase HSP70.