| 引用本文: | 宋志前,夏磊,曹玉娜,曾林燕,张琳琳,魏征,刘振丽.同时测定乳香中杂质和挥发油含量[J].中国现代应用药学,2013,30(4):388-390. |
| SONG Zhiqian,XIA Lei,CAO Yu’na,ZENG Linyan,ZHANG Linlin,WEI Zheng,LIU Zhenli.Determination of Essential Oil and Impurities in Olibanum[J].Chin J Mod Appl Pharm(中国现代应用药学),2013,30(4):388-390. |
|
| 摘要: |
| 目的 建立同时测定乳香中挥发油和杂质含量的方法。方法 以能将乳香中杂质以外的部分全部溶解为目标，对提取溶剂数量和种类进行考察。同时，以杂质以外的部分全部溶解所需时间为指标，对比超声提取和回流提取，确定最佳溶剂、溶剂用量和回流时间。结果 单纯一种溶剂不能将乳香中杂质以外的部分全部溶解。建立的挥发油和杂质测定方法为：取一定量乳香，加入水，采用水蒸气蒸馏法提取挥发油，滤过，药渣再加入乳香量1.25倍的丙酮，回流提取5 min，滤过，不溶残渣即为杂质。收集的11个批次乳香及制乳香中挥发油含量范围为1.25%~4.24%，杂质为0.75%~2.4%。乳香挥发油含量比制乳香高，杂质含量无明显差异。结论 建立了一种同时测定乳香与制乳香中挥发油和杂质含量的方法，为质量标准的制订提供了参考。 |
| 关键词: 乳香 制乳香 杂质 挥发油 测定方法 |
| DOI： |
| 分类号: |
| 基金项目:国家重大科技专项课题(2009ZX09103-314) |
|
| Determination of Essential Oil and Impurities in Olibanum |
|
SONG Zhiqian1, XIA Lei2, CAO Yu’na3, ZENG Linyan1, ZHANG Linlin1, WEI Zheng3, LIU Zhenli1
|
|
1.The Institute of Basic Theory, China Academy of Chinese Medical Sciences, Beijing 100700, China;2.Tianjin Pharmaceuticals Research Oganization Co. Ltd., Tianjin 300384, China;3.School of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
|
| Abstract: |
| OBJECTIVE To establish an effective method for simultaneously determining the contents of essential oil and impurities in Olibanum. METHODS The amount and species of extraction solution were investigated with a goal of dissolving all the components except impurites in Olibanum. Meanwhile, supersonic extraction and reflux extraction were compared with a goal of the time to dissolve all the part except impurities, finally, the optimal solution, amount and reflux time were determined. RESULTS All the part except impurities in Olibanum could not be dissolved in only one solution. The method for determining the essential oils and impurities was as follow: a certain amount of Olibanum were extracted with water by steam distillation to obtain essential oils, filter, then the residue were refluxed with 1.25 times actone for 5 min, filter, and the residue were impurites. The content ranges of essential oil in eleven batches of Olibanum were from 1.25% to 4.24%, and those of impurities were from 0.75% to 2.4%. The contents of essential oil in Olibanum were higher than that in processed products, and there was no obvious difference in the contents of impurities. CONCLUSION A method was established to simultaneously determine the contents of essential oils and impurities both in Olibanum and their processed products, and a reference was provided for the establishment of quality standard. |
| Key words: Olibanum processed Olibanum impurities essential oil determination |
