| 引用本文: | 付青姐,李明春,柳迎华.辐射对小鼠脾脏损伤及香菇多糖的保护作用[J].中国现代应用药学,2013,30(6):606-609. |
| FU Qingjie,LI Mingchun,LIU Yinghua.Protective Effects of Lentinan Against Spleen Injury in Mice to Radiation[J].Chin J Mod Appl Pharm(中国现代应用药学),2013,30(6):606-609. |
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| 摘要: |
| 目的 研究辐射对小鼠脾脏的损伤以及香菇多糖对其保护作用。方法 建立小鼠低剂量长期辐射的损伤模型。小鼠随机分为5组：对照组、模型组、给药剂量分别为0.02，0.1，0.5 mg·mL-1的香菇多糖组。测定各组小鼠的脾脏系数、MTT法测定小鼠脾脏淋巴细胞的增殖情况，HE染色法分析脾脏组织的病理变化，用透射电镜法分析脾脏细胞的超微结构。结果 与对照组相比，低剂量慢性辐射能降低小鼠的脾脏系数、抑制脾脏淋巴细胞的增殖，对小鼠脾脏造成病理损伤，破坏脾脏细胞的超微结构。香菇多糖0.1，0.5 mg·mL-1组可明显抑制辐射造成的脾脏的上述损伤。结论 香菇多糖对辐射造成的脾脏损伤具有保护作用。 |
| 关键词: 辐射 香菇多糖 脾脏 |
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| Protective Effects of Lentinan Against Spleen Injury in Mice to Radiation |
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FU Qingjie, LI Mingchun, LIU Yinghua
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The Chinese People’s Liberation Army Hospital 401, Qingdao 266071, China
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| Abstract: |
| OBJECTIVE To study the radiation damage on mouse spleen and the protective effects of lentinan. METHODS Mouse model of chronic injury was made by exposing to 60Co g-ray which were divided into five groups: control group, model group, different doses of lentinan groups(0.02, 0.1, 0.5 mg·mL-1). After exposing, spleen coefficient, ability of lymphocyte proliferation were checked by MTT, changes on pathology and ultramicrostructure were respectively determined. RESULTS Compared with control group, spleen coefficient, ability of lymphocyte proliferation, pathology and ultramicrostructure were damaged by 60Co g-ray in model group. Whereas, the 0.1, 0.5 mg·mL-1 lentinan groups were uninfluenced by radiation. CONCLUSION Lentinan normalizes the injury of spleen. |
| Key words: radiation lentinan spleen |
