HPLC同时测定西归中阿魏酸和佛手柑内酯的含量

Simultaneous Determination of Bergapten and Ferulic Acid in Seselopsis by HPLC

关键词:  西归  阿魏酸  佛手柑内酯  含量测定  高效液相色谱法

Key words:  Seselopsis  ferulic acid  bergapten  content determination  HPLC

摘要:

目的 建立同时测定西归药材中阿魏酸和佛手柑内酯含量的方法。方法 采用色谱柱为PhenomeneX Gemini-NX C18 110A柱(4.6 mm×250 mm，5 μm)；流动相为甲醇-0.05%磷酸水溶液，梯度洗脱；流速为1.0 mL·min^-1；检测波长为322 nm；柱温为30 ℃。结果 阿魏酸在0.020 1~0.603 μg、佛手柑内酯在0.020 3~0.609 μg内线性关系良好；阿魏酸和佛手柑内酯的平均回收率分别为96.8%(RSD=2.0%)和98.7%(RSD=2.9%)。结论 本方法首次建立了同时测定西归中阿魏酸和佛手柑内酯的HPLC分析方法，结果准确，重复性好，可作为西归药材的质量评价方法。

Abstract:

OBJECTIVE To simultaneous determine the content of ferulic acid and bergapten in Seselopsis. METHODS The chromatographic separation was performed on a PhenomeneX Gemini-NX C18 110A (250 mm×4.60 mm, 5μm). The mobile phase was consisted of methanol-0.05% phosphoric acid with gradient elute, the flow rate was 1.0 mL·min^-1. The detection wavelength was 322 nm and the column temperature was 30 ℃. RESULTS Good linear relationship between the peak area and content of ferulic acid and bergapten was achieved when the amount was within 0.020 1-0.603 μg, 0.020 3-0.609 μg; the recoveries were 96.8% and 98.7%; RSD were 2.0% and 2.9%. CONCLUSION This is the first time to simultaneous determine ferulic acid and bergapten in Seselopsis. The method is accurate, reproducible and suitable to be used for quality assessment of Seselopsis.