HPLC波长切换法测定不同产地水红花子中花旗松素和槲皮素的含量

HPLC Wavelength Switching Method for the Determination of Different Origin Polygonum Orientale Taxifolin and Quercetin Content

关键词:  水红花子  花旗松素  槲皮素  波长切换

Key words:  fructus polygoni orientalis  taxfolin  quercetin  wavelength switchiing

摘要:

目的 建立HPLC测定水红花子中花旗松素和槲皮素含量的方法，为水红花子质量标准的研究提供科学依据。方法 用高效液相色谱波长切换法同时测定花旗松素和槲皮素的含量，色谱条件为Agilent TC-C₁₈色谱柱(4.6 mm×250 mm，5 mm)，以A相0.1%磷酸水和B相甲醇∶乙腈(60∶40)为流动相梯度洗脱，其中B相为甲醇与乙腈按60∶40固定体积比例配制的混合有机相。流速：1 mL·min^-1，检测波长：0~42 min为290 nm，42~49 min为371 nm，温度：25 ℃。结果 此方法线性良好，花旗松素和槲皮素的平均加样回收率分别为99.89%，99.22%；RSD分别为2.03%，3.00%。结论 不同产地的水红花子药材中花旗松素和槲皮素的含量存在较大差异；该方法稳定，重复性好，操作简单，可作为水红花子药材的质量控制方法，也为合理利用水红花子这一资源提供依据。

Abstract:

OBJECTIVE To establish the method for determining the content of taxfolin and quercetin in fructus polygoni for its quality standards to provide the scientific basis for the study. METHODS The Agilent TC-C₁₈ Column(4.6 mm×250 mm, 5 μm) was used, phase A was 0.1% phosphoric acid and phase B was methanol∶acetonitrile (60∶40) as the mobile phase, gradient elution, where phase B was mixed by methanol and acetonitrile in 60∶40 volume ratio of mixed organic phase. The flow rate was 1.0 mL·min^-1, the detection wavelength 290 nm in 0-42 min, 371 nm in 42-49 min, the column temperature was 25 ℃. RESULTS The isolation effect among taxfolin and quercetin showed good linear correlation, the average recoveries were 99.89%, 99.22%; RSD were 2.03%, 3.00%. CONCLUSION There have a great differences in contents of taxifolin and quercitrin among the samples from different origins. The method is simple, accurate and with good reproducibility. Which could be used as fructus polygoni orientalis quality control of medicines, it also provide a scientific reference for the rational use of fructus polygoni orientalis.