山银花不同物候期总皂苷的含量测定

Determination of Total Tritorpenoid Saponin in Different Buds Stages of Lonicerae Flos

关键词:  山银花  总皂苷  灰毡毛忍冬皂苷乙  比色法

Key words:  Lonicerae Flos  total triterpenoid saponin  macranthoidin B  colorimetric method

摘要:

目的 建立山银花药材中总皂苷含量测定方法。方法 采用分光光度法，以香草醛-高氯酸为显色剂，在477 nm处测定不同物候期山银花药材中总皂苷的含量。结果 灰毡毛忍冬皂苷乙在0.501~6.012 μg·mL^-1内呈良好的线性关系，平均加样回收率为100.1%。山银花花蕾不同发育期样品中总皂苷含量差异明显，以灰毡毛忍冬皂苷乙计，总皂苷含量在8.00%~21.41%内均有分布。结论 山银花整个花期皂苷类成分差异明显，其中银花期含量最高，米蕾期最低。本实验为山银花药材的采收加工提供了数据支撑。

Abstract:

OBJECTIVE To establish the determination method of total triterpenoid saponins in Lonicerae Flos. METHODS The colorimetric method was used with vanillin-perchloric acid as the chromogenic reagent. The detection wavelength was set at 477 nm. RESULTS The linear range of macranthoidin B was 0.501-6.012 μg·mL^-1 and the average recovery was 100.1%. The contents of total triterpenoid saponin in the samples collected from the different stages of the buds of L. macranthoides were various and fluctuated from 8.00%-21.41%. CONCLUSION The obvious difference of the total content of triterpenoid saponin is observed in six development stages of the whole flower season of L. macranthoides. The content of total teripenoid saponin in the silver phase is highest and the bud phase is lowest. The present investigation provides the scientific evidences for the optimal selection of collection and process of Lonicerae Flos.