三七及其炮制品对血虚模型大鼠的补血益气作用比较

Comparative of Notoginseng Radix Et Rhizome and Its Processed Products on Enriching Blood and Supplementing Qi of Rats with Blood-deficiency

关键词:  三七  炮制品  血虚  补血益气

Key words:  Notoginseng Radix et Rhizome  processed  blood deficiency  blood-enriching and supplementing Qi

摘要:

目的 评价生三七及其不同炮制品对血虚模型大鼠的药效作用，比较三七炮制前后在补血益气功效上的差异。方法 大鼠皮下注射2%乙酰苯肼生理盐水溶液制备血虚模型，分别灌胃生三七水提物、生三七醇提物、蒸三七水提物、蒸三七醇提物、油炸三七水提物、油炸三七醇提物，观察给药后各组间面温、肛温、血液学指标[红细胞计数(RBC)、红细胞压积(HCT)]、凝血及出血时间、尾部微循环血流量。结果 蒸三七水提物和醇提物能明显增加血虚模型大鼠尾部微循环血流量(P<0.01)；蒸三七水提物可升高大鼠RBC、HCT(P<0.01)，缩短凝血时间(P<0.05)；蒸三七醇提物可升高面温、肛温(P<0.05)。油炸三七水提物可升高大鼠RBC、HCT，升高大鼠面温、肛温(P<0.05)；油炸三七醇提物能升高面温、增加微循环血流量(P<0.05)。生三七水提物和醇提物能明显缩短大鼠凝血时间和出血时间(P<0.01或P<0.05)；生三七水提物可升高RBC(P<0.05)，生三七醇提物可增加微循环血流量(P<0.05)。结论 熟三七(蒸三七、油炸三七)在提高面温、肛温、促进造血作用优于生三七，说明熟三七具有益气补血作用，即三七“熟补”。其中，蒸三七改善微循环效果较好，生三七止血作用最明显。

Abstract:

OBJECTIVE To evaluate the medicinal effect of different processing products of Notoginseng Radix et Rhizome on animal model of blood-deficiency, and to make comparison of blood-enriching effect among those products to screen out the optimal product. METHODS The 2% acetyl phenyl hydrazine solution was subcutaneously injected into rats for establishing the blood-deficiency model. Each group were separately treated with different processing product of Notoginseng Radix et Rhizome by oral administration. Donkey-hide gelatin was used in the positive control group. The parameters of facial rectal-temperature, complete blood count (CBC), blood microcirculation, and blood coagulation time/bleeding time were estimated. RESULTS The aqueous and ethanol extracte of steamed Notoginseng Radix et Rhizoma could significantly improve the rat blood microcirculation(P<0.01); the aqueous extract could raise the rat RBC and HCT(P<0.01), decrease blood coagulatio time(P<0.05); the ethanol extract could raise facial and rectal temperature(P<0.05). The aqueous extracte of fried Notoginseng Radix et Rhizoma could significantly raise RBC, HCT, facial and rectal temperature(P<0.01); the ethanol extracte of fried Notoginseng Radix et Rhizoma could also raise facial temperature, and improve the rat blood microcirculation(P<0.05). The aqueous and ethanol extracte of raw product Notoginseng Radix et Rhizoma could significantly significantly decrease blood coagulatio time/bleeding time(P<0.01 or P<0.05); the aqueous extract could raise the rat RBC(P<0.05); the ethanol extract could improve the rat blood microcirculation(P<0.05). CONCLUSION The processing products of Notoginseng Radix et Rhizome has better effect of blood-enriching than the raw product, but has no better effect in the blood coagulation time/bleeding time.