HPLC测定桑叶中绿原酸、芦丁和木犀草苷含量

Determination of Chlorogenic Acid, Rutin, and Luteolin Glycosides in Mulberry Leaves by HPLC

关键词:  高效液相色谱法  桑叶  绿原酸  芦丁  木犀草苷

Key words:  HPLC  mulberry leaves  chlorogenic acid  rutin  luteolin glycosides

摘要:

目的 建立同时测定桑叶中绿原酸、芦丁和木犀草苷含量的方法。方法 样品用 70%乙醇溶液提取，采用Phenomenex Luna C₁₈色谱柱(4.6 mm×250 mm，5 μm)分离；流动相：0.1%磷酸-乙腈，梯度洗脱；流速：1.0 mL·min^-1；检测波长：348 nm。结果 绿原酸、芦丁、木犀草苷分别在3.048~152.4，1.059~52.96，1.437~71.85 μg·mL^-1内具有良好的线性关系；加标回收率分别为100.4%，98.3%，98.7%；RSD分别为1.7%，2.0%，1.9%(n=9)。结论 该方法简单易行，准确性和重复性好，可用于桑叶的质量控制。

Abstract:

OBJECTIVE To determine the content of chlorogenic acid, rutin and luteolin glycosides in mulberry leaves by HPLC. METHODS The sample was extracted by 70% ethanol and separated on a Phenomenex Luna column(4.6 mm×250 mm, 5 μm). 0.1% Phosphoric acid and acetonitrile was used as mobile phase with gradient elution at flow rate of 1.0 mL·min^-1 The detection wavelength was set at 348 nm. RESULTS The linear ranges of chlorogenic acid, rutin and luteolin glycosides were 3.048-152.4, 1.059-52.96 and 1.437-71.85 μg·mL^-1. And the average recoveries were 100.4%, 98.3% and 98.7% with the RSD of 1.7%, 2.0%, 1.9%(n=9), respectively. CONCLUSION This method is simple, rapid and with good repeatability, and can be used for the quality control of mulberry leaves.