| 引用本文: | 崔錾,韩冠英,马寅达,郭斌.萝藦果壳多糖脱蛋白方法研究[J].中国现代应用药学,2013,30(8):856-859. |
| CUI Zan,HAN Guanying,MA Yinda,GUO Bin.Study on Deproteinization Methods of Polysaccharide from Metaplexis Japonica Nutshell[J].Chin J Mod Appl Pharm(中国现代应用药学),2013,30(8):856-859. |
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| 摘要: |
| 目的 确定萝藦果壳多糖脱蛋白的最优方法。方法 以蛋白脱除率和多糖损失率为指标，比较氯仿-正丁醇法(Sevag法)、三氯乙酸法(TCA法)、木瓜蛋白酶-Sevag法和木瓜蛋白酶-TCA法。结果 Sevag法、TCA法、木瓜蛋白酶-Sevag法、木瓜蛋白酶-TCA法的蛋白脱除率分别为73.14%，71.55%，86.03%，75.33%，多糖损失率分别为24.40%，25.68%，8.47%，17.13%。结论 木瓜蛋白酶-Sevag法是除去萝藦果壳多糖中蛋白的最佳方法。 |
| 关键词: 萝藦果壳 多糖 脱蛋白 |
| DOI： |
| 分类号:R285.5 |
| 基金项目: |
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| Study on Deproteinization Methods of Polysaccharide from Metaplexis Japonica Nutshell |
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CUI Zan1, HAN Guanying2, MA Yinda2, GUO Bin2
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1.Liaoning Medical University, Jinzhou 121001, China;2.The No.1 Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, China
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| Abstract: |
| OBJECTIVE To elect the best deproteinization method of polysaccharide from Metaplexis Japonica nutshell. METHODS The protein was removed by the methods of Sevag, TCA, papain-sevag and papain-TCA. The rate of deproteinization and the loss rate of polysaccharide were detected. RESULTS The percentage of deproteinization of Sevag, TCA, papain-Sevag and papain-TCA were 73.14%, 71.55%, 86.03%, 75.33% respectively and the loss rate of polysaccharide were 14.40%, 9.22%, 8.47%, 7.13% respectively. CONCLUSION The best deproteinization method of polysaccharide from Metaplexis Japonica nutshell was the papain-Sevag method. |
| Key words: Metaplexis japonica nutshell polysaccharide deproteinization |
