水蛭对血瘀证家兔血脂代谢及相关基因表达的影响

Effect of Hirudo on Blood Metabolism and Its Related Gene Expression in Blood Stasis Syndrome Rabbits

关键词:  水蛭  血瘀证  血脂代谢  LDL-R  ApoE

Key words:  Hirudo  blood stasis syndrome  lipid metabolism  LDL-R  ApoE

摘要:

目的 观察水蛭对实验性血瘀证家兔血脂代谢及其相关基因表达的影响。方法 采用复合因素(饥饿+高脂饲料+注射肾上腺素)建立血瘀证模型，造模结束后将实验动物随机分成5组，高中低水蛭剂量组、模型对照组、空白对照组，三个剂量组分别按0.30，0.15，0.075g·kg^-1给血瘀证家兔灌胃30 d。分别于造模结束时、灌胃第10，20，30天心脏取血，检测血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)含量；采用荧光实时定量PCR技术检测家兔肝脏低密度脂蛋白受体(LDL-R)、载脂蛋白E(ApoE) mRNA的表达。结果 与模型组相比，水蛭能显著降低血清中TC、TG、LDL-C水平(P<0.05)，能显著上调血瘀证家兔肝脏中LDL-R mRNA、ApoE mRNA的表达(P<0.05)。结论 水蛭具有调节血瘀证家兔血脂代谢的作用，其机制可能与上调LDL-R基因和ApoE基因转录水平有关。

Abstract:

OBJECTIVE To observe the effects of Hirudo on blood metabolism and its related gene expression in blood stasis syndrome rabbits. METHODS The blood stasis syndrome rabbit’s models induced by complex factors(starvation+ high-fat feeding+adrenaline) were established. Model rabbits randomly divided into five groups, rabbits in Hirudo groups were daily administered with Hirudo(0.30, 0.15, 0.075 g·kg^-1) for 30 days, and rabbits in the normal and model groups were daily administered with 0.9% NaCl. The serum TC, TG, LDL-C in each different treated rabbit group were determined after models established, 10, 20, 30 d administration, respectively. The mRNA expressions of LDL-R and ApoE in liver tissue were quantified by Realtime-PCR. RESULTS Compared with normol group, Hirudo groups could remarkably decrease the levels of serum TC, TG, LDL-C(P<0.05). The result of realtime-PCR demonstrated that the Hirudo could remarkly increase the mRNA expressions of LDL-R and ApoE in liver tissue of rabbit’s model group. CONCLUSION Hirudo has the effect of regulating lipld metabolism of blood stasis syndrome rabbits, the mechanism is likely related to increasing LDL-R and ApoE mRNA expression.