黄芩素联合紫杉醇对人肺癌细胞的生长抑制作用

Effect of Baicalein Combined Paclitaxel on Proliferation of Human Lung Carcinoma Cell

关键词:  黄芩素  紫杉醇  肺癌细胞  凋亡

Key words:  baicalein  paclitaxel  lung cancer cell  apoptosis

摘要:

目的 探讨黄芩素联合紫杉醇对人肺癌细胞株QG-56的影响。方法 以不同浓度的黄芩素、紫杉醇分别组成单药组和联合用药组，另设不加药的空白对照组，分别作用于QG-56细胞24，48，72和96 h，观察细胞数量和形态的变化，MTT法检测各组对QG-56细胞的增殖抑制作用，用流式细胞仪检测各组细胞凋亡率的改变。结果 不同浓度的各实验组作用后细胞数量明显减少、形态不规则，部分细胞核固缩和胞质减少，而对照组细胞生长状态良好。黄芩素、紫杉醇单用与联用均能抑制QG-56细胞增殖，呈剂量-时间依赖效应，联用组细胞抑制率较单药组明显增高，差异有统计学意义(P<0.05)。黄芩素、紫杉醇单用与联用均能诱导QG-56细胞凋亡，联合组更为明显(P<0.05)。结论 黄芩素可显著抑制人肺癌细胞株QG-56细胞生长，并能有效增强紫杉醇对人肺癌QG-56细胞的增殖抑制作用；两者具有协同作用。

Abstract:

OBJECTIVE To explore the effect of baicalein combined with paclitaxel on human lung carcinoma cell line QG-56. METHODS QG-56 cells were treated with baicalein, paclitaxel alone or their combination, with the untreated cells used as control. After 24, 48, 72, 96 hours of treatment, the cell growth condition was observed by invert microscope and inhibitation rate was studied by MTT assay. The apoptosis was analyzed by flow cytometry. RESULTS The cell population decreased and shape, size changed after treating with different concentration of experimental groups. Baicalein and paclitaxel used alone or in combination both inhibited the proliferation of QG-56 cells. The inhibition ratio of their combination was more higher(P<0.05), and a synergistic effect of the two agents was noted in their combined action(P<0.05). Combined treatment of the cells resulted in significantly higher apoptsis rate than that alone(P<0.05). CONCLUSION Baicalein can significantly inhibit QG-56 cell growth. It can act synergistically with paclitaxel to inhibit the tumor proliferation of QG-56 cells．A synergistic effect is observed between them.