猫爪草总皂苷体外抗人非小细胞肺癌NCI-H460细胞活性研究

Study on the in Vitro Activity of Ranunculus Ternati Radix Saponins on Non-Small Cell Lung Cancer Cell of NCI-H460

关键词:  猫爪草总皂苷  人非小细胞肺癌NCI-H460细胞  细胞凋亡  细胞周期

Key words:  Ranunculus Ternati Radix saponins  NCI-H460  apoptosis  cell cycle

摘要:

目的 观察猫爪草总皂苷体外对人非小细胞肺癌NCI-H460细胞活性的影响。方法 采用系统溶剂法提取猫爪草总皂苷，³H-TdR掺入法观察猫爪草总皂苷对NCI-H460细胞增殖的影响；流式细胞仪检测其对NCI-H460细胞凋亡和周期的影响。结果 猫爪草总皂苷对NCI-H460细胞增殖有较好的抑制作用，呈现较好的量效关系，可促进NCI-H460细胞的早期凋亡，细胞周期出现G₀/G₁期的阻滞。结论 猫爪草总皂苷能较好的抑制NCI-H460细胞增殖，其机制可能与诱导凋亡、G₀/G₁期阻滞有关。

Abstract:

OBJECTIVE To observe the in vitro activity of Ranunculus Ternati Radix saponins(RRTS) on non-small cell lung cancer(NSCLC) cell of NCI-H460. METHODS Ranunculus Ternati Radix saponins was extracted by the technique of solvent-refining. Effect of different concentrations of Ranunculus Ternati Radix saponins on cell NCI-H460 were tested by ³H-TdR incorporation method. The cell cycle phase and apoptosis were inspected by flow cytometry. RESULTS The results showed that RRTS could inhibit the growth of NCI-H460 cell, showing a dose-effect curve. It could also promote the early apoptosis of cell NCI-H460 and arrest the cell cycle in G₀/G₁ phase. CONCLUSION Ranunculus Ternati Radix saponins can inhibit the proliferation of cell NCI-H460, its mechanism is probably related to apoptosis induction and blockage of cell cycle in G₀/G₁ phase.