HPLC测定宫血宁胶囊中重楼皂苷Ⅰ、Ⅱ、Ⅵ及Ⅶ的含量

Determination of Polyphyllin Ⅰ, Ⅱ, Ⅵ and Ⅶ in Gongxuening Capsule by HPLC

关键词:  宫血宁胶囊  高效液相色谱法  重楼皂苷Ⅰ  重楼皂苷Ⅱ  重楼皂苷Ⅵ  重楼皂苷Ⅶ

Key words:  Gongxuening capsule  HPLC  polyphyllin Ⅰ  polyphyllinⅡ  polyphyllinⅥ  polyphyllin Ⅶ

摘要:

目的 建立高效液相测定宫血宁胶囊中重楼皂苷Ⅰ、Ⅱ、Ⅵ及Ⅶ含量的方法。方法 色谱柱为Phenomenex Luna C₁₈ (4.6 mm×250 mm，5 μm)；流动相为乙腈(A)-水(B)梯度洗脱；流速为1.0 mL·min^-1；柱温为25 ℃；检测波长为203 nm。结果 重楼皂苷Ⅰ、Ⅱ、Ⅵ及Ⅶ分别在2.024~14.168，2.010~14.07，2.016~14.112及2.032~14.224 μg·mL^-1内与峰面积呈良好的线性关系，r分别为0.999 8，0.999 7，0.999 8和0.999 8；平均加样回收率分别为99.0%(RSD=0.29%)，99.4% (RSD=1.13%)，99.4%(RSD=0.58%)，100.3%(RSD=0.95%)。结论 本法简便快捷，具有良好的精密性和稳定性，测定结果可靠，可用于本产品的质量控制。

Abstract:

OBJECTIVE　To establish an HPLC method for determination of polyphyllin Ⅰ, Ⅱ, Ⅵ and Ⅶ in Gongxuening capsule. METHODS Phenomenex Luna C₁₈ column (4.6 mm×250 mm, 5 μm) was used. Mobile phase was acetonitrile(A)- water(B) with gradient elute. The flow rate was 1.0 mL·min^-1, the column temperature was 25 ℃, the detection wavelength was 203 nm. RESULTS The content of polyphyllin Ⅰ, Ⅱ, Ⅵ and Ⅶ had a good linear relationship in the range of 2.024- 14.168, 2.010-14.07, 2.016-14.112 and 2.032-14.224 μg·mL^-1, and r was 0.999 8, 0.999 7, 0.999 8 and 0.999 8, respectively. The average recoveries of them were 99.0%(RSD=0.29%), 99.4%(RSD=1.13%), 99.4%(RSD=0.58%), 100.3(RSD=0.95%), respectively. CONCLUSION The methods is simple and quick, which has good precision and stability, the result is reliable and can be used for quality control of Gongxuening capsule.