HPLC快速筛查抗风湿类中成药及保健品中非法添加的10种化学药

Screening for 10 Anti-rheumatism Chemical Components in Health Food and Chinese Traditional Medicine by HPLC

关键词:  中成药  保健品  高效液相色谱法  快速筛查

Key words:  traditional Chinese medicines  health food  HPLC  rapid detection

摘要:

目的 建立中成药、保健食品中非法添加的10种抗风湿类化学成分的快速测定方法。方法 采用高效液相色谱法进行初步筛查。色谱柱：Diamonsil C₁₈(4.6 mm×250 mm，5 μm)，流动相：乙腈-0.02 mol·L^-1醋酸铵溶液，梯度洗脱；流速：1.0 mL·min^-1，检测波长：240 nm。结果 10种化学药得到了完全分离，检出限<2 ng。结论 本方法操作简单、快速，灵敏度高，能满足快速筛查抗风湿类中成药及保健品中非法添加化学药的需要。

Abstract:

OBJECTIVE To establish a method for rapid detection of 10 anti-rheumatism chemical components adding illegally in health food and traditional Chinese medicine. METHODS Using HPLC to conduct the preliminary screening. The Diamonsil C₁₈(4.6 mm×250 mm, 5 μm) was used. The mobile phase consisted of acetonitrile-0.02 mol·L^-1 ammonium acetate with linear gradient elution at a flow rate of 1.0 mL·min^-1, the detection wavelength was 240 nm. RESULTS These ten components were separated completely, and the LODs were <2 ng.CONCLUSION The method is simple, sensitive and quickly to detect chemical components in health food and Chinese Traditional Medicine.