| 引用本文: | 刘慧璐,冯建勇,王增尚,吴晨,张末初,周胜,刘江云,郝丽莉.高色价栀子黄的精制工艺研究[J].中国现代应用药学,2013,30(12):1315-1319. |
| LIU Huilu,FENG Jianyong,WANG Zengshang,WU Chen,ZHANG Mochu,ZHOU Sheng,LIU Jiangyun,HAO Lili.Preparative Separation of Crocins with High Color Value from Gardenia Fruits[J].Chin J Mod Appl Pharm(中国现代应用药学),2013,30(12):1315-1319. |
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| 摘要: |
| 目的 对栀子黄进行进一步分离制备，以获得高色价的西红花总苷及其中主要单体成分。方法 采用中压反相柱层析技术，对栀子黄进行进一步分离制备，采用液相色谱检测西红花苷-1含量，采用紫外可见光谱检测色价及其吸光度比值。结果 制备工艺可有效去除绿原酸等杂质，精制后的西红花总苷样品中西红花苷-1含量为60.8%，色价高达756；并可分离获得西红花苷-1(1)、西红花苷-2(2)、西红花苷-3(3)等化合物。结论 该制备方法简便、高效、成本较低，可为栀子黄的精制及产业化生产提供参考。 |
| 关键词: 栀子 栀子黄 西红花苷 分离 反相柱层析 |
| DOI： |
| 分类号:R284.1；R917.101 |
| 基金项目:苏州大学大学生创新创业训练计划项目(2012xj065) |
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| Preparative Separation of Crocins with High Color Value from Gardenia Fruits |
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LIU Huilu, FENG Jianyong, WANG Zengshang, WU Chen, ZHANG Mochu, ZHOU Sheng, LIU Jiangyun, HAO Lili
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College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, China
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| Abstract: |
| OBJECTIVE To establish an efficient separation process to afford gardenia yellow and crocins with high color value. METHODS The process was conducted using reversed-phase medium pressure liquid chromatography. A high pressure liquid chromatography - photodiode array method was applied for qualitative and quantitative analysis of crocin-1. Moreover, the color value was also evaluated by UV method. RESULTS After the reversed-phase separation, the content of crocin-1 in refined product reached 60.8%, with the color value to be 756. Crocin-1(1), crocin-2(2) and crocin-3(3) could be separated either with similar procedure. CONCLUSION The process developed is highly efficient, low-cost and compact, which affords a potential approach for industrial production of crocins with improved quality. |
| Key words: Gardenia jasminoides gardenia yellow crocin separation reversed-phase chromatography |
