大孔树脂分离纯化刺五加中紫丁香苷、刺五加苷E和异嗪皮啶的工艺研究

Study on Separation and Purification of Syringin, Aleutheroside E and Isofraxidin from Acanthopanax Senticosus by Macroporous Resin

关键词:  刺五加  紫丁香苷  刺五加苷E  异嗪皮啶  大孔树脂

Key words:  Acanthopanax senticosus  syringin  eleutheroside E  isofraxidin  macroporous resin

摘要:

目的 研究大孔树脂分离纯化刺五加中紫丁香苷、刺五加苷E和异嗪皮啶的工艺。方法 比较了4种不同类型的大孔树脂对刺五加中紫丁香苷、刺五加苷E和异嗪皮啶的吸附解吸性能，确定适宜的树脂类型和最佳纯化工艺条件。结果 AB-8型大孔树脂对紫丁香苷、刺五加苷E和异嗪皮啶的吸附性能及解吸效果较好。最佳工艺条件为上样浓度：0.5 g·mL^-1，吸附流速：2 BV·h^-1，上样量：5 BV，洗脱剂：30%的乙醇，用量：11 BV，洗脱流速：1 BV·h^-1。纯化后的终产品中紫丁香苷、刺五加苷E和异嗪皮啶的含量分别为3.13%，1.82%和0.42%。结论 AB-8型大孔树脂用于同时富集紫丁香苷、刺五加苷E和异嗪皮啶效果最佳，是一种理想的分离纯化介质。

Abstract:

OBJECTIVE To study the separation and purification technology of syringin, eleutheroside E and isofraxidin from Acanthopanax senticosus by macroporous resin. METHODS The adsorption and desorption rate of four types of macroporous resins was compared, so as to determine the proper kind of resin and the best condition for purifying. RESULTS The adsorption and isolation effect of AB-8 resin was much better. The optimal conditions were as follow: the concentration of sample solution was 0.5 g·mL^-1, the adsorption flow rate was 2 BV·h^-1, the maximum loading amount was 5 BV, the eluent was 30% ethanol, the dosage was 11 BV and the elution flow rate was 1 BV·h^-1. Under the optimal conditions, the content of syringin, eleutheroside E and isofraxidin in purified powder was 3.13%, 1.82% and 0.42%, respectively. CONCLUSION AB-8 is an ideal resin with the best enrichment for separating and purifying syringin, eleutheroside E and isofraxidin simultanously.