| 引用本文: | 江松敏,陈智心,蔡琳.FGF21对大鼠肝癌模型中TGF-β信号通路的影响[J].中国现代应用药学,2014,31(3):261-265. |
| JIANG Songmin,CHEN Zhixin,CAI Lin.Effect of FGF21 on TGF-β Signaling Pathway in Rat Liver Cancer[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(3):261-265. |
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| 摘要: |
| 目的 研究FGF21通过TGF-β/Smads信号通路延缓肝癌发生发展的分子机制。方法 采用Western blot法检测各组大鼠肝癌组织的TGF-β1, TGF-β RⅡ, Smad 2,3,4,7的蛋白表达水平，采用Q-PCR法检测各组大鼠肝癌组织的TGF-β1, TGF-β RⅡ, Smad 2,3,4,7的mRNA表达水平。结果 在不同FGF21浓度下，TGF-β1，TGF-β RⅡ，Smad 2,3,4,7表达水平不同(P<0.05)。结论 FGF21通过促进TGF-β/Smads信号通路中的信号分子或受体的表达，或者通过下调此信号通路的阻断分子而抑制肿瘤发生。 |
| 关键词: FGF21 TGF-β1 Smad |
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| 基金项目:浙江省教育厅科研项目(Y200907097)；温州市科技计划项目(Y20090257) |
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| Effect of FGF21 on TGF-β Signaling Pathway in Rat Liver Cancer |
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JIANG Songmin1, CHEN Zhixin2, CAI Lin2
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1.Ruian People’s Hospital, Ruian 325200, China;2.School of Pharmacy, Wenzhou Medical University, Wenzhou 325035, China
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| Abstract: |
| OBJECTIVE To research the possible molecular mechanisms of FGF21 via TGF-b/Smads signal pathway to delay the development of liver cancer. METHODS Detect the protein expressions of TGF-β1, TGF-β RⅡ, Smad 2,3,4,7 of respective rats cancer tissue with Western blot and detect the mRNA expression of TGF-β1, TGF-β RⅡ, Smad 2,3,4,7 of respective rats cancer tissue with Q-PCR. RESULTS The expressions of TGF-β1, TGF-β RⅡ, Smad 2,3,4,7 varied with the different concentrations of FGF21(P<0.05). CONCLUSION By promoting TGF-β/Smads signaling pathway in the expression of signaling molecules or receptors, or by decreasing the blocking of this signaling pathway, tumor was inhibited by FGF21. |
| Key words: FGF21 TGF-β1 Smad |
