| 引用本文: | 刘新义,李健和,易利丹,胡雄彬,唐甜甜,向大雄,叶祖光.PEG化葛根素降低红细胞溶血的体内研究[J].中国现代应用药学,2014,31(4):402-405. |
| LIU Xinyi,LI Jianhe,YI Lidan,HU Xiongbin,TANG Tiantian,XIANG Daxiong,YE Zuguang.Study on Reducing Hemolysis by PEGylated Puerarin in Vivo[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(4):402-405. |
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| 摘要: |
| 目的 探讨PEG修饰技术降低葛根素诱导红细胞溶血的体内研究。方法 采用葛根素注射液和PEG化葛根素给予家兔每天等剂量静脉注射，连续7 d，每天取血3 mL，进行血细胞计数分析；7 d实验结束后再取血，检测红细胞渗透脆性和红细胞膜流动性。结果 血细胞计数分析结果表明，PEG化葛根素组家兔的血细胞计数与空白对照组没有显著性差异，而葛根素注射液组家兔出现了血管内溶血现象；红细胞渗透脆性和红细胞膜流动性结果表明，PEG化葛根素可以降低红细胞渗透脆性和增强红细胞膜流动性。结论 PEG修饰技术可以减轻葛根素诱导血管内溶血的不良反应，更安全。 |
| 关键词: PEG化葛根素 葛根素注射液 血细胞计数分析 红细胞渗透脆性 红细胞膜流动性 |
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| 基金项目:湖南省自然科学青年基金(14JJ3038) |
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| Study on Reducing Hemolysis by PEGylated Puerarin in Vivo |
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LIU Xinyi1, LI Jianhe1, YI Lidan1, HU Xiongbin1, TANG Tiantian1, XIANG Daxiong1, YE Zuguang2
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1.Department of Pharmacy, the Second Xiangya Hospital of Central South University, Changsha 410013, China;2.China Academy of Chinese Medical Sciences, Beijing 100700, China
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| Abstract: |
| OBJECTIVE To investigate the reducing hemolysis effect induced by PEGylation of puerarin via in vivo. METHODS Rabbits were once-daily given the same dose of puerarin injection and PEGylated puerarin intravenously for 7 days, 3 mL blood was taken every day and used for complete blood counts analysis. At the end of 7 days of experiment, blood was taken for the determination of osmotic fragility of RBC and erythrocyte membrane fluidity. RESULTS Blood counts analysis indicated that there was no significant difference between PEGylated puerarin group and blank control group. Osmotic fragility of RBC and erythrocyte membrane fluidity indicated that PEGylated puerarin could reduce osmotic fragility of RBC and enhance erythrocyte membrane fluidity. CONCLUSION PEGylation technology could reduce intravascular hemolysis induced by puerarin, which is more safe. |
| Key words: PEGylated puerarin puerarin injection blood counts analysis osmotic fragility of RBC erythrocyte membrane fluidity |
