牡荆苷对大鼠骨髓间充质干细胞成骨分化的影响

Effect of Vitexin on the Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells

关键词:  牡荆苷  骨髓间充质干细胞  成骨分化

Key words:  vitexin  mesenchymal stem cells  osteogenic differentiation

摘要:

目的 探讨牡荆苷对体外培养大鼠骨髓间充质干细胞成骨分化的影响。方法 采用MTT法检测牡荆苷对骨髓间充质干细胞生存率的影响，以对硝基苯磷酸盐法检测碱性磷酸酶活性，茜素红法染色评价钙化结节形成。实时荧光定量PCR检测runt-related transcription factor 2(Runx2)和osterix(Osx)表达。结果 牡荆苷在0.1~50 μmol·L^-1浓度内对骨髓间充质干细胞生存率无显著影响。牡荆苷能明显提高骨髓间充质干细胞内碱性磷酸酶活性，并能促进钙化结节形成。牡荆苷也上调Runx2和Osx基因表达。结论 牡荆苷可促进骨髓间充质干细胞向成骨细胞方向分化，其作用可能与上调Runx2和Osx基因表达有关。

Abstract:

OBJECTIVE To investigate the effect of vitexin on osteogenic differentiation of rat bone marrow mesenchymal stem cells(MSCs). METHODS Methylthiazolyl tetrazolium(MTT) assay was applied to determine the viability of MSCs. Alkaline phosphatase(ALP) activity was detected using p-nitrophenyl phosphate assay. Nodule formation of calcium was observed with alizarin red stain. The genes expression of runt-related transcription factor 2 (Runx2) and osterix (Osx) was detected by real time PCR. RESULTS Vitexin at concentration from 0.1 to 50 μmol·L^-1 had no effect on viability of MSCs. Vitexin effectively stimulated the activity of ALP in MSCs and increased the nodule formation of calcium. Vitexin also up-regulated the expression of Runx2 and Osx mRNA. CONCLUSION Vitexin improves the osteogenic differentiation of MSCs, which may be related with the up-regulation of the expression of Runx2 and Osx.