| 引用本文: | 覃俏峰,蓝晓庆,温庆伟,刘婷,陆东,韦红言,宋景政.HPLC-ELSD测定复方罗汉果止咳冲剂中罗汉果皂苷Ⅴ的含量[J].中国现代应用药学,2014,31(7):850-852. |
| QIN Qiaofeng,LAN Xiaoqing,WEN Qingwei,LIU Ting,LU Dong,WEI Hongyan,SONG Jingzheng.Determination of Mogroside V in Fufang Luohanguo Zhike Granule by HPLC-ELSD[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(7):850-852. |
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| 摘要: |
| 目的 建立HPLC-ELSD测定止咳冲剂中的罗汉果皂苷复方罗汉果V的含量的方法。方法 色谱条件是AlltimaTM-C18色谱柱(4.6 mm×250 mm，5 μm)；流动相乙腈-水(23∶77)，柱温30 ℃，ELSD检测条件，漂移管温度为105 ℃，气流流量为2.0 L·min-1。结果 罗汉果皂苷Ⅴ进样量在1.608~9.648 μg内时，进样量的对数与峰面积的对数呈良好的线性关系，回归方程为Y=1.634 3X+12.591(r=0.999 7)，平均回收率为101.36%，RSD为1.29%。结论 该方法准确、专属性强，可用于测定复方罗汉果止咳冲剂中罗汉果皂苷Ⅴ的含量。 |
| 关键词: 复方罗汉果止咳冲剂 罗汉果皂苷Ⅴ 高效液相色谱-蒸发光散射检测器 |
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| 基金项目:广西创新计划专项经费项目(2014CXJHA02)；南宁市科学研究与技术开发计划项目(20141060，20133163) |
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| Determination of Mogroside V in Fufang Luohanguo Zhike Granule by HPLC-ELSD |
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QIN Qiaofeng1, LAN Xiaoqing2, WEN Qingwei2, LIU Ting2, LU Dong2, WEI Hongyan2, SONG Jingzheng2
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1.The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, China;2.PuraPharm (Nanning) Pharmaceuticals Co., Ltd., Nanning 530007, China
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| Abstract: |
| OBJECTIVE To establish an HPLC-ELSD method for the determination of morgtoside V in Fufang Luohanguo Zhike granule. METHODS AlltimaTM-C18 column(4.6 mm×250 mm, 5 μm), mobile phase: acetonitrile-water(23∶77), column temperature: 30 ℃. ELSD condition: temperature of drift tube: 105 ℃, flow rate of gas: 2.0 mL·min-1.RESULTS Morgtoside V was linear in the range of 1.608-9.648 μg, with regression equation of Y=1.634 3X+12.591(r=0.999 7), the average recovery was 101.36%(RSD=1.29%). CONCLUSION The established method is accurate, specific and reliable for the determination of morgtoside V in Fufang Luohanguo Zhike granule. |
| Key words: Fufang Luohanguo Zhike granule morgtoside V HPLC-ELSD |
