| 引用本文: | 吴巧攀,乔洪翔,何厚洪,胡江宁,吴健,姚建标,王如伟.银杏叶渣中多糖的提取及其抗氧化活性研究[J].中国现代应用药学,2014,31(1):9-13. |
| WU Qiaopan,QIAO Hongxiang,HE Houhong,HU Jiangning,WU Jian,YAO Jianbiao,WANG Ruwei.Extraction of polysaccharides from residue of Ginkgo Biloba and study on anti-oxidant activity[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(1):9-13. |
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| 摘要: |
| 目的 研究从银杏叶渣中提取多糖的工艺,及其抗氧化活性。方法 以多糖提取率为评价指标,通过苯酚-硫酸法检测,经单因素试验得到最佳工艺,所得银杏多糖进行还原能力和清除DPPH自由基的试验。结果 最佳工艺条件为：料液比1:7,提取时间2h,提取2次,醇沉浓度90%,醇沉1h。银杏多糖具有较强的还原能力和清除DPPH自由基的能力。结论 该工艺简便可行,稳定可靠,适于工业生产,所得多糖具有较强的抗氧化活性。 |
| 关键词: 银杏叶渣 多糖 提取 自由基清除 抗氧化 |
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| 基金项目:(1) 国家十二五重大新药创制专项(编号：2011ZX09203-001-18)； (2) 浙江省重点实验室和中试基地计划项目(编号：2009E10009) |
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| Extraction of polysaccharides from residue of Ginkgo Biloba and study on anti-oxidant activity |
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WU Qiaopan1, QIAO Hongxiang2, HE Houhong2, HU Jiangning2, WU Jian2, YAO Jianbiao2, WANG Ruwei2
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1.Zhejiang University of Traditional Chinese Medicine;2.Zhejiang Institute of TCM＆Natural Drug Co.Ltd
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| Abstract: |
| Objective: To develop the optimal extraction technology of polysacchrides from residue of Ginkgo Biloba , and conduct anti-oxidant tests. Methods: Taking the extraction ratio of polysaccharides for evaluation index and detected by phenol-sulfuric acid method, the optimal technology is obtained through single factor experiments. And experiments are taken on reducing activity and scavenging activity against DPPH. Results :The optimal extraction conditions are material-to-liquid ratio of 1:7, extraction time of 2 hours, 2 times, alcohol precipitation concentration of 90%, alcohol precipitation time of 1 hours . The polysaccharides obtained have a rather good reducing activity and scavenging activity against DPPH . Conclusion :The technology is convenient and workable, stable and dependable, which is suitable for industrial manufacture. The polysaccharides obtained have strong anti-oxidant activities. |
| Key words: residue of Ginkgo Biloba polysaccharides extraction scavenging free radicals anti-oxidant activity |
