| 引用本文: | 王金明,王石健,张晓芬,杨赛成,王彬辉.HPLC测定人血清中多索茶碱的浓度[J].中国现代应用药学,2014,31(8):988-991. |
| WANG Jinming,WANG Shijian,ZHANG Xiaofen,YANG Saicheng,WANG Binhui.Determination of Doxofylline in Human Serum by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(8):988-991. |
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| 摘要: |
| 目的 建立高效液相色谱测定多索茶碱血药浓度的方法。方法 用乙醚提取血样，以卡马西平为内标进行检测。色谱柱采用Waters C18(3.9 mm×150 mm，5 μm)，流动相为甲醇-水(35∶65)，流速为0.6 mL·min?1，检测波长为273 nm，柱温为30 ℃。结果 多索茶碱血清浓度在0.7~28 μg·mL?1内线性关系良好，线性方程为Y=0.354 9X+0.003 9(r=0.999 7)，方法回收率为100.4%，日内、日间RSD分别为1.10%~3.87% 和2.11%~4.86%(n=5)。结论 该方法快速、简便、准确、安全，可用于临床多索茶碱血药浓度的测定。 |
| 关键词: 高效液相色谱法 多索茶碱 血药浓度 |
| DOI： |
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| 基金项目:台州学院基金项目(2012PY45) |
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| Determination of Doxofylline in Human Serum by HPLC |
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WANG Jinming1,2, WANG Shijian2, ZHANG Xiaofen2, YANG Saicheng2, WANG Binhui2
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1.Zhejiang Chinese Medical University, Hangzhou 310053, China;2.Department of Pharmacy, Municipal Hospital of Taizhou, Taizhou 318000, China
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| Abstract: |
| OBJECTIVE To establish an HPLC method for determination of doxofylline in human serum. METHODS Ethyl ether was used as the exacted solvent, with carbamazepine as the internal standard. The column was Waters-C18 (3.9 mm×150 mm, 5 μm). The mobile phase was methanol-water(35∶65) and the flow rate was 0.6 mL·min?1. The detection wavelength was 273 nm. The column temperature was kept at 30 ℃. RESULTS It showed a good linear range of 0.7-28 μg·mL?1 for doxofylline, the linear regression equation was Y=0.354 9X+0.003 9(r=0.999 7). The methodological recovery was 100.4%, the intra-day RSD and the inter-day RSD were 1.10%-3.87% and 2.11%-4.86%(n=5). CONCLUSION The method is proved to be rapid, simple, accurate and safe. It is suitable for determination of concentration of doxofylline in clinic. |
| Key words: HPLC doxofylline serum drug concentration |
