哮喘大鼠肺组织Toll样受体3和9的表达及布地奈德的影响

方丽; 陈培英; 童夏生; 陈琪; 范广民; 王恩智

引用本文:	方丽,陈培英,童夏生,陈琪,范广民,王恩智.哮喘大鼠肺组织Toll样受体3和9的表达及布地奈德的影响[J].中国现代应用药学,2014,31(7):805-809.
	FANG Li,CHEN Peiying,TONG Xiasheng,CHEN Qi,FAN Guangmin,WANG Enzhi.Effect of Budesonide on Expression of Toll Like Receptor 3 and 9 in Rat Asthma Model[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(7):805-809.

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哮喘大鼠肺组织Toll样受体3和9的表达及布地奈德的影响
方丽¹, 陈培英¹, 童夏生², 陈琪³, 范广民⁴, 王恩智⁵
1.诸暨市人民医院儿科，浙江绍兴 311800;2.台州市中西医结合医院儿科，浙江台州 317000;3.台州中心医院检验科，浙江台州 317000;4.台州中心医院，病理科，浙江台州 317000;5.台州市中西医结合医院，检验科，浙江台州 317000

摘要:

目的观察哮喘大鼠肺组织中Toll样受体3(TLR3)和TLR9的表达及对布地奈德的影响，探讨TLRs在哮喘炎症机制中的作用。方法建立大鼠哮喘模型, 随机分成哮喘组、对照组和布地奈德组，免疫组织化学法检测肺组织TLR3和TLR9的表达。结果哮喘组(OD值：0.201±0.034)和布地奈德组(OD值：0.195±0.043)肺组织TLR3的光密度值显著高于对照组(OD值：0.144±0.039)( P <0.05)，布地奈德组与哮喘组差异无统计学意义。哮喘组(OD值：0.236±0.022)和布地奈德组(OD值：0.231±0.023)肺组织TLR9的光密度值均显著低于对照组(OD值：0.271±0.025)( P <0.05)；布地奈德组与哮喘组差异无统计学意义；肺组织TLR3和TLR9蛋白的表达水平无显著相关性(n =26， r =-0.153)。结论哮喘大鼠肺组织TLR3蛋白表达升高，TLR9蛋白的表达则反之，TLRs的表达过度或不足可能是哮喘气道炎症的重要原因之一。布地奈德的抗炎作用可能主要不是通过TLR3和TLR9的途径实现。

关键词: 哮喘 Toll样受体糖皮质激素免疫组织化学

DOI：

分类号:R965.2

基金项目:温岭市科技局基金资助项目(2009-2-55)

Effect of Budesonide on Expression of Toll Like Receptor 3 and 9 in Rat Asthma Model

FANG Li¹, CHEN Peiying¹, TONG Xiasheng², CHEN Qi³, FAN Guangmin⁴, WANG Enzhi⁵

1.Department of Pediatrics, People’s Hospital of Zhuji, Shaoxing 311800, China;2.Taizhou Integrated Traditional and Western Medicine Hospital, Department of Pediatrics, Taizhou 317000, China;3.Taizhou Central Hospital, Department of Clinical Laboratory,Taizhou 318000, China;4.Taizhou Central Hospital, Department of Pathology, Taizhou 318000, China;5.Taizhou Integrated Traditional and Western Medicine Hospital, Department of Clinical Laboratory, Taizhou 317000, China

Abstract:

OBJECTIVE To investigate the potential roles of Toll like receptor(TLR) in pathogenesis of asthma inflammation, and the expression of TLR 3 and 9 in rat asthma model. METHODS The rats were randomly and averagely divided into asthma, control and budesonide-treated group. Expressions of TLR3 and TLR9 protein were detected by immunohistochemical method. RESULTS Expressions of TLR3 protein in lung tissure in asthma group(optical density: 0.201±0.034) and in budesonide-treated group(optical density: 0.195±0.043) were significantly higher than that in control group(optical density: 0.144±0.039)(all P <0.05). While there was no significant difference of TLR3 protein expression between budesonide-treated and asthma group. Dramaticly, expressions of TLR9 protein in lung tissure in asthma group(0.236±0.022 optical density) and budesonide-treated group(optical density: 0.231±0.023) were significantly lower than that in control group(optical density: 0.271±0.025)(all P <0.05). Moreover, there was no significant difference of TLR9 protein expression between in budesonide-treated and asthma group. Furthermore, there was no significant correlation between levels of TLR3 and TLR9 in lung tissue( n =26, r =-0.153, P >0.05). CONCLUSION Level of TLR3 increased in asthmatic asthma lung tissue group. On the other hand, level of TLR9 decreased. TLRs may act as an important role in asthma exacerbation, and the anti- inflammation function of TLRs may not mainly work through TLR3 and TLR9 path way.

Key words: asthma Toll like receptor glucocorticoid immunohistochemistry