加杨树叶中3种黄酮类成分的含量测定

Determination of Three Flavonoids in Populus Canadensis Moench. Leaves

关键词:  加杨树叶  槲皮素  高良姜素  白杨素  高效液相色谱法

Key words:  Populus canadensis Moench. leaves  quercetin  galangin  chrysin  HPLC

摘要:

目的 建立同时测定加杨树叶中的槲皮素、高良姜素、白杨素含量的分析方法。方法 采用高效液相色谱法，色谱柱Agilent TC C₁₈柱(4.6 mm×250 mm，5 μm)，流动相：甲醇-0.1%甲酸，梯度洗脱：0~5 min，甲醇25%→55%，5~25 min，甲醇55%→65%，25~35 min，甲醇65%→75%；流速：1.0 mL·min^-1；检测波长：264 nm；柱温：30 ℃。结果 加杨树叶中槲皮素、高良姜素、白杨素分离效果良好，线性范围分别为3.7~120，2.5~80和3.125~100 μg·mL^-1；平均加样回收率分别为99.0%，100.1%，101.4%。结论 该方法准确可靠、结果稳定、重复性好，可为评价加杨树叶的质量提供依据。

Abstract:

OBJECTIVE To establish an analytical method for simultaneous determination of quercetin, galangin and chrysin in Populus canadensis Moench. leaves. METHODS The determination was performed on Agilent TC C₁₈ column (4.6 mm×250 mm, 5 μm) with the mobile phase consists of methanol-0.1% formic acid at a flow rate of 1.0 mL·min^-1. The following gradient elution was used: the content of methanol was 25% to 55% from 0 to 5 min, 55% to 65% from 5 to 25 min and 65% to 75% from 25 to 35 min. The detection wavelength was set at 264 nm, and the column temperature was 30 ℃. RESULTS This method had a good separation performance for quercetin, galangin and chrysin in poplar leaves, and the linear range were 3.7-120, 2.5-80 and 3.125-100 μg·mL^-1, respectively; the average recovery rates were 99%, 100.1% and 101.4%, respectively. CONCLUSION This method is accurate, reliable with good stability and repeatability which provides the evidence for quality evaluation for poplar leaves.