心脑宁片对脑缺血大鼠乳酸、LDH、ATP酶活力的影响

Effect of Xinnaoning Tablets on Lactic Acid, LDH and ATP in Cerebral Ischemia Rats

关键词:  心脑宁片  大鼠脑缺血模型  乳酸  乳酸脱氢酶  ATP酶活力

Key words:  Xinnaoning tablets  cerebral ischemia  lactic acid  LDH, ATPase activity

摘要:

目的 研究心脑宁片对大鼠脑缺血模型脑匀浆乳酸(lactic acid，LD)、乳酸脱氢酶(lactate dehydrogenase，LDH)水平及三磷酸腺苷(adenosine-triphosphate，ATP)酶活力的影响。方法 Wistar大鼠随机分为7组，每组12只：空白对照组，高、中、低剂量心脑宁片组、尼莫地平组、脑安片组和羧甲基纤维素钠组，测定脑匀浆中蛋白含量、LD、LDH、ATP酶水平。结果 大鼠脑缺血模型造模成功。与模型组相比，各剂量心脑宁片均可显著降低脑匀浆LD水平(P<0.01)，升高脑匀浆LDH活力(P<0.01)，并可显著升高脑匀浆Na⁺-K⁺-ATPase(P<0.01)、Mg²⁺-ATPase和Ca²⁺-ATPase活力(P<0.01或P<0.05)结论 心脑宁片具有明显改善脑缺血作用。

Abstract:

OBJECTIVE To study the effect of Xinnaoning tablets on lactic acid, LDH and ATPase activity effect in cerebral ischemia rats. METHODS Wistar rats were randomly divided into 7 groups: control group, model, high- middle- and low-dose of Xinnaoning tablets group, nimodipine group, Naoan tablets group and 0.5% CMC group. The content of brain homogenate protein, LD, LDH, ATPase levels were determined. RESULTS The model of cerebral ischemia rats were established successfully. Compared with model group, high-, middle-, and low- dose of Xinnaoning tablets could significantly decrease LD, raise LDH, and elevate brain homogenates of Na⁺-K⁺-ATPase, Mg²⁺-ATPase activity and Ca²⁺-ATPase activity (P<0.01 or P<0.05). CONCLUSION Xinnaoning tablets have significantly improve effect on cerebral ischemia.