人参皂苷Rd对高糖所致HMC表达TGF-β1的影响

In Vitro Effects of Ginsenoside Rd on Proliferation and Expression of TGF-β1 in Human Mesangial Cells Induced by High Glucose

关键词:  人参皂苷Rd  转化生长因子β1  人肾小球系膜细胞

Key words:  ginsenoside Rd  TGF-β1  human mesangial cells

摘要:

目的 考察人参皂苷Rd对高糖条件下人肾小球系膜细胞(human mesangial cells，HMC)增殖及对HMC分泌的转化生长因子β1(TGF-β1)表达的影响。方法 将人肾小球系膜细胞分为正常血糖组、高糖对照组、高糖+药物组(5组，人参皂苷Rd浓度分别为6.25，12.5，25，50，100 μg·mL^-1)，分别于24，48，72 h用MTT法检测细胞增殖情况；实时定量荧光PCR检测TGF-β1的mRNA的表达；ELISA试剂盒检测HMC分泌TGF-β1的量。结果 高糖对HMC的增殖无明显影响，但能显著促进HMC分泌TGF-β1的功能，并上调TGF-β1 mRNA的表达，与正常血糖组比较具有统计学意义；而不同浓度的人参皂苷Rd对高糖所致HMC合成、分泌TGF-β1的功能均有不同程度的抑制作用，其中，浓度为12.5，25，50，100 μg·mL^-1的人参皂苷Rd效果显著，具有显著性差异( P <0.05)，且能下调TGF-β1 mRNA的表达。结论 人参皂苷Rd可以减少高糖状态下HMC合成和分泌的TGF-β1蛋白的量以及下调TGF-β1 mRNA的表达。

Abstract:

OBJECTIVE To investigate the effect of ginsenoside Rd on proliferation and expression of transforming growth factor β1(TGF-β1) in human mesangial cells (HMC) induced by high glucose. METHODS Seven groups were set up including normal-sugar group(NG), high glucose group(HG), high glucose plus ginsenoside Rd of five different dosage(6.25, 12.5, 25, 50, 100 μg·mL^-1) groups. After the cells treated respectively for 24, 48, 72 h, cell proliferation was measured by MTT. Expression of TGF-β1 was detected by enzyme-linked immuno sorbent assay (ELISA). Expression of TGF-β1 mRNA was detected by RT-PCR. RESULTS Compared with those in the NG, HMC which cultured in high glucose had higher levels of TGF-β1 and TGF-β1 mRNA. Ginsenoside Rd of different dosage inhibited secretion of TGF-β1 of HMC in HG, dosage of 12.5, 25, 50, 10 μg·mL^-1 had the strongest effect( P <0.05). It also reduced TGF-β1 mRNA expression. CONCLUSION Ginsenoside Rd can reduce TGF-β1 mRNA expression and secretion of TGF-β1 protein quantity of HMC which is cultured in high glucose conditions.