精制冠心颗粒复方中4味药材HPLC指纹图谱研究

Study on HPLC Fingerprint of Four Kinds of Drugs of Jingzhi Guanxin Keli Fufang

关键词:  精制冠心颗粒复方  指纹图谱  HPLC  丹参酮ⅡA

Key words:  Jingzhi Guanxin Keli Fufang  fingerprint  HPLC  tanshinone ⅡA

摘要:

目的 利用HPLC建立精制冠心颗粒复方中4味药材指纹图谱。方法 运用Agilent 1100 DAD-HPLC高效液相色谱仪，采用Inertsil ODS-3(4.6 mm×250 mm，5 μm)色谱柱，乙腈-0.1%磷酸梯度洗脱，流速1.0 mL·min^-1，检测波长270 nm，柱温30 ℃，进样量：10 μL，对精制冠心颗粒复方四味药材进行试验。结果 10批精制冠心颗粒复方中4味药材中共获得22个共有峰，不同批次样品相似度均>0.90，方法精密度、稳定性和重复性良好。结论 采用HPLC建立的精制冠心颗粒复方中4味药材指纹图谱，方法稳定、可靠、简便，色谱图展现的各峰分离度较好，特征明显，可做为精制冠心颗粒复方真伪鉴别标准。

Abstract:

OBJECTIVE To establish the chromatographic fingerprint of Jingzhi Guanxin Keli Fufang by HPLC. METHODS HPLC was performed on Jingzhi Guanxin Keli Fufang using Agilent 1100 DAD-HPLC. Inertsil ODS-3 column (4.6 mm×250 mm, 5 μm) was used; the mobile phase was acetonitrile-0.1% phosphoric acid for gradient elution; the flow rate was 1 mL·min^-1; the detection wavelength was 270 nm; the column temperature was 30 ℃, and inject volume was 10 μL. RESULTS Twenty-two HPLC common peaks were acquired in the 10 batches of four kinds of drugs of Jingzhi Guanxin Keli Fufang, and their similarities were >0.90. Good precision, stability and repetition were showed. CONCLUSION A reliable method is provided for the quality identification of Jingzhi Guanxin Keli Fufang. And the chemical components of different germplasms of Jingzhi Guanxin Keli Fufang are different from each other; selection of germplasm is important in quality control of Jingzhi Guanxin Keli Fufang.