HPLC测定芩暴红止咳胶囊中东莨菪内酯、黄芩苷、黄芩素和杜鹃素的含量

Determination of Scopoletin, Baicalin, Baicalein and Farrerol in Qinbaohong Zhike Capsule by HPLC

关键词:  芩暴红止咳胶囊  高效液相色谱法  东莨菪内酯  黄芩苷  黄芩素  杜鹃素  含量测定

Key words:  Qinbaohong Zhike capsule  HPLC  scopoletin  baicalin  baicalein  farrerol  content determination

摘要:

目的 采用HPLC测定芩暴红止咳胶囊中东莨菪内酯、黄芩苷、黄芩素、杜鹃素的含量。方法 Eclipse XDB-C₁₈色谱柱(4.6 mm×250 mm，5 μm)，流动相：甲醇(A)-1%冰醋酸溶液(B)，梯度洗脱，流速：0.8 mL·min^-1；检测波长：295 nm；柱温：30 ℃。结果 东莨菪内酯线性范围为1.27~124.70 μg，r=0.999 9，加样回收率为98.3%(RSD=1.1%)；黄芩苷线性范围为8.07~538.20 μg，r=1.000 0，样品加样回收率为98.0%(RSD=1.2%)；黄芩素线性范围为1.12~111.60 μg，r=1.000 0，加样回收率为98.5%(RSD=0.9%)；杜鹃素线性范围为1.06~106.40 μg，r=1.000 0，加样回收率为99.0%(RSD=1.4%)。结论 本方法简便、准确，重复性好，专属性高，可用于制剂的质量控制。

Abstract:

OBJECTIVE To establish a method for determining the content of scopoletin, baicalin, baicalein and farrerol in Qinbaohong Zhike capsule. METHODS An Eclipse XDB-C₁₈ column (4.6 mm×250 mm, 5 μm) was used with methanol (A)-1% acetic acid (B) as the mobile phase at the flow rate of 0.8 mL·min^-1, and the UV-detection wavelength was set at 295 nm with the column temperature of 30 ℃. RESULTS Scopoletin showed a good linear relationship at the range of 1.27-124.70 μg (r=0.999 9), the average recovery was 98.3% with RSD of 1.1%. Baicalin showed a good linear relationship at the range of 8.07-538.20 μg(r=1.000 0), the average recovery was 98.0% with RSD of 1.2%. Baicalein showed a good linear relationship at the range of 1.12-111.60 μg(r=1.000 0), the average recovery was 98.5% with RSD of 0.9%. Farrerol showed a good linear relationship at the range of 1.06-106.40 μg(r=1.000 0), the average recovery was 99.0% with RSD of 1.4%. CONCLUSION This method is simple, accurate and reproducible. It can be reliable for the quality control of Qinbaohong Zhike Capsule.