| 引用本文: | 王桂玲,房建强,王克娜,赵雪梅.Box-Behnken效应面法优化龙葵中总生物碱的提取工艺[J].中国现代应用药学,2014,31(10):1202-1207. |
| WANG Guiling,,FANG Jianqiang,WANG Kena,ZHAO Xuemei.Optimization of Extraction Technology for Total Alkaloids from Solanum Nigrum L. By Box-Behnken Design[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(10):1202-1207. |
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| 摘要: |
| 目的 优选龙葵中总生物碱的提取工艺。方法 采用单因素试验结合Box-Behnken 效应面法，以总生物碱提取率为指标，考察乙醇体积分数、料液比、提取时间、提取次数等因素对提取工艺的影响，采用酸性染料比色法，利用紫外分光光度计测定总生物碱的含量。结果 确定龙葵中总生物碱提取的最佳工艺条件：乙醇体积分数为63.72%，料液比为1∶41.23，提取时间为54.93 min，提取2次，总生物碱提取率为1.40 mg·g-1。结论 Box-Behnken 效应面法用于龙葵中总生物碱提取工艺条件的优选是可行的，模型预测效果较好。 |
| 关键词: 龙葵 总生物碱 提取工艺 Box-Behnken效应面法 |
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| 基金项目:泰山医学院学校青年科学基金(2010ZRQN013) |
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| Optimization of Extraction Technology for Total Alkaloids from Solanum Nigrum L. By Box-Behnken Design |
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WANG Guiling,, FANG Jianqiang, WANG Kena, ZHAO Xuemei
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Taishan Medical University, Tai’an 271016, China
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| Abstract: |
| OBJECTIVE To optimize the extraction technology for total alkaloids from Solanum nigrum L. by Box-Behnken design. METHODS Based on the results of single-factor experiment, Box-Behnken experimental design combined with response surface methodology(RSM) was employed, with the ethanol concentration(A), solid-liquid ratio(B), extraction time(C) and extraction times(D) as the independent variables. The response variable was the amount of total alkaloids. Acid dye colorimetry and UV spectrophotometer were used to detect the content of total alkaloid in Solanum nigrum L. RESULTS The optimization processing parameters was as follows: A, B, C and D levels of 63.72%, 41.23 times, 54.93 min and extract twice, respectively. The content of total alkaloid was 1.40 mg·g-1. CONCLUSION Box-Behnken experimental design is feasible, and highly predictive. |
| Key words: Solanum nigrum L. total alkaloids extraction process Box-Behnken design |
