小春花治疗肺心病模型大鼠肺动脉高压的研究

Sceptridium Ternatum For the Treatment of Pulmonary Arterial Hypertension in Rats with Pulmonary Heart

关键词:  肺心病  肺动脉高压  小春花

Key words:  pulmonary heart disease  pulmonary arterial hypertension  Sceptridium Ternatum

摘要:

目的 观察小春花对肺心病模型大鼠肺动脉高压(pulmonary arterial hypertension，PAH)的影响。方法 120只SD大鼠随机分为正常对照组、模型组、西地那非组和小春花提取物高、中、低剂量组，每组20只。采用野百合碱(60 mg·kg^-1)皮下注射建立大鼠肺心病PAH模型；连续灌胃给药21 d。采用右心导管法测定平均右心室压(mean right ventricular pressure，mRVP)、平均肺动脉压(mean pulmonary arterial pressure，mPAP)和最大右心室压(max right ventricular pressure，max RVP)，处死动物测定称取肺湿重(lung wet weight，WW)、右心室游离壁(right ventricu tar free wall mass，RV)、左心室加心室间隔(left ventricular pus septal，LV+S)重量，计算肺指数(lung index，LI)和右心肥厚指数(right ventricular hypertrophy index，RVHI)。结果 模型组大鼠mRVP、mPAP和max RVP显著高于正常对照组( P <0.001)；与模型组比较，小春花高、中剂量组大鼠mPAP、mRVP和max RVP均显著降低( P <0.001~0.05)；小春花高剂量组大鼠RV、LV+S、WW、LI均显著低于模型组( P <0.001~0.01)。结论 小春花对野百合碱诱导的大鼠肺心病PAH有一定的干预作用。

Abstract:

OBJECTIVE To observe the effect of Sceptridium Ternatum on pulmonary arterial hypertension in rats with pulmonary heart disease. METHODS All of 120 SD rats were randomly divided into control, model, sildenafil, Sceptridium Ternatum low-dose, middle-dose and high-dose drug groups, 20 rats in each group. The animal model were established by intraperitoneally injecting moncrotaline of 60 mg·kg^-1. Rats in control and model groups were given saline by gavage for 21 d. The mean right ventricular pressure(mRVP), mean pulmonary arterial pressure(mPAP), and max RVP were measured by using right cardiac catheterization. The rats were then sacrificed for determination of lung wet weight(WW) and the weight of right ventricule(RV) and left ventricule pus septum(LV+S). Lung index(LI) and right ventricular hypertrophy index(RVHI) were calculated. RESULTS The mRVP, mRAP, max RVP in model group were higher than those of the control group( P <0.001), and those parameters in the middle- and high-dose drug groups were lower than those of the model group( P <0.001-0.05). The RV, LV+S, WW, RVHI and LI in the high-dose drug group were smaller than those of the model group( P <0.001-0.01). CONCLUSION Sceptridium Ternatum may have some effect on pulmonary arterial hypertension induced by monocrotaine in rats.