| 引用本文: | 宋晓凯,曹志凌,郭雷,李志华.醉香含笑心材挥发性成分GC-MS分析及抑制MDA-MB-231细胞生长与诱导其凋亡作用[J].中国现代应用药学,2014,31(8):911-915. |
| SONG Xiaokai,CAO Zhiling,GUO Lei,LI Zhihua.GC-MS Analysis of Volatile Components from Trunk of Michelia Macclurei Dandy. and the Inhibition of ETMMD on Growth of MDA-MB-231 Cell Lines and Its Apoptosis-inducing[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(8):911-915. |
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| 摘要: |
| 目的 分析醉香含笑心材提取物(extract of trunk of Michelia macclurei Dandy.，ETMMD)的挥发性成分，并考察其对人乳腺癌细胞MDA-MB-231的体外生长抑制作用及诱导其凋亡的作用。方法 水蒸气蒸馏法提取醉香含笑心材的挥发性成分，用气-质联用(GC-MS)技术进行分析，并用峰面积归一化法测定各成分相对含量；采用MTT法及流式细胞术分别检测 6.25，12.50，25.00 μg·mL?1的ETMMD对MDA-MB-231细胞体外生长抑制作用及诱导其凋亡的作用。结果 共检出42个色谱峰，确定了其中41种化合物，占该挥发性成分总量的99.73%。ETMMD对MDA-MB-231细胞的抑制率呈浓度依赖关系。结论 ETMMD的挥发性成分以倍半萜烯及不饱和酮、不饱和酯类为主，此外还包括高级脂肪酸酯，以及一些甾体类化合物。ETMMD能明显抑制MDA-MB-231细胞的生长。浓度为6.25，12.50，25.00 μg·mL?1的ETMMD可明显诱导人乳腺癌细胞 MDA-MB-231的凋亡。 |
| 关键词: 醉香含笑心材挥发性成分 气相色谱-质谱分析 MDA-MB-231细胞 生长抑制 凋亡诱导 |
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| 基金项目:国家自然科学基金资助项目(20872110) |
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| GC-MS Analysis of Volatile Components from Trunk of Michelia Macclurei Dandy. and the Inhibition of ETMMD on Growth of MDA-MB-231 Cell Lines and Its Apoptosis-inducing |
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SONG Xiaokai1, CAO Zhiling1, GUO Lei2, LI Zhihua1,3
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1.College of Chemical Technology, Huaihai Institute of Technology, Lianyungang 222005, China;2.College of Marine Sciences, Huaihai Institute of Technology, Lianyungang 222005, China;3.School of Chemical Engineering and Technology, China University of Mining and Technology, Xuzhou 221116, China
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| Abstract: |
| OBJECTIVE To analysis the volatile components from extract of trunk of Michelia macclurei Dandy. (ETMMD), and explore the inhibition of ETMMD on growth of MDA-MB-231 cell lines in vitro. METHODS The volatile components from ETMMD were extracted with steam distillation method and analysized by GC-MS. The relative content of each component was calculated by peak area normalization. The inhibiting action of ETMMD on growth of MDA-MB-231 cell lines in vitro were examined by MTT assay, and the apoptosis-inducing of ETMMD on MDA-MB-231 cell lines was explored by flow cytometry assay. RESULTS There were 42 peaks were detected, in which 41 compounds were identified, which made up 99.73% of the total volatile components. From MTT assay, the inhibition rates of ETMMD on growth of MDA-MB-231 cell lines in vitro were observed in concerntration-dependent manner, and the apoptosis-inducing of ETMMD on MDA-MB-231 cell lines was observed by flow cytometry. CONCLUSION The main components in the volatile components are sesquiterpene and unsaturated ketones, unsaturated esters, unsaturated fatty acid ester and steroids, etc. ETMMD can significantly inhibit the growth of MDA-MB-231 cell lines in vitro and significantly induce MDA-MB-231 cell lines apoptosis. |
| Key words: volatile components of trunk of Michelia macclurei Dandy GC-MS analysis MDA-MB-231cell inhibition of growth apoptosis-inducing |
