地黄块根中化学成分变化规律及与其组织结构相关性研究

Correlative Analysis Between Change Regularity of Chemical Composition and Organizational Structure in Rehmannia Glutinosa Libosch

关键词:  地黄  化学成分  石蜡切片  动态积累  主成分分析

Key words:  Rehmannia glutinosa Libosch  chemical composition  paraffin section  dynamic accumulation  the principal components analysis

摘要:

目的 研究地黄在生长过程中化学成分含量的变化，确定地黄主要化学成分动态积累的规律及与其组织结构变化的相关性，确定最佳采收期。方法 采用HPLC对地黄中梓醇、毛蕊花糖苷进行含量测定；采用RP-HPLC-RID对地黄中单、寡糖进行含量测定；采用硫酸-苯酚法对地黄中多糖含量进行测定；采用氨基酸自动分析仪测定地黄中氨基酸含量；采用SPSS软件对各成分进行主成分分析；通过制作石蜡切片、徒手切片观察地黄组织结构变化；并采用SPSS软件对其化学成分含量与其组织结构间的相关性进行分析。结果 地黄85-5中梓醇含量与木质部比例、皮层比例有显著相关性，多糖含量与韧皮部比例有显著相关性，水苏糖含量与木质部比例、韧皮部占木质部比例、皮层比例、分泌细胞数目有显著相关性；北京3号中氨基酸含量与皮层比例、分泌细胞数目有显著相关性。结论 地黄块根中各化学成分均在10下旬至11月上旬含量较高，此时块根韧皮部比例最小，木质部比例最大，与传统地黄采收期相吻合。

Abstract:

OBJECTIVE To study the changes of chemical composition in Rehmannia glutinosa during the growth process. And to determine the correlation between the accumulation trends of main chemical composition and changes of organizational structure. To determine the best harvesting time of Rehmannia glutinosa. METHODS Content of catalpol and verbascoside in Rehmannia glutinosa were determined by HPLC; monosaccharide and oligosaccharide in Rehmannia glutinosa were determined by the method of RP-HPLC-RID; polysaccharide in Rehmannia glutinosa were determined by the method of phenol-sulfuric; amino acids in Rehmannia glutinosa were determined by automatic amino acid analyzer. SPSS software were used to have the principal components analysis for each chemical composition to observe the changes of organizational structure through the paraffin section and freehand section. SPSS software were used to analysis the correlation between the content of chemical composition and organizational structure. RESULTS There were significant correlations between the content of catalpol and the proportion of the xylem, the cortex; the content of polysaccharide and the proportion of the phloem;the content of stachyose and the proportion of the xylem, the phloem of xylem, the cortex, the numbers of secretory cell in 85-5. There were significant correlations between the content of amino acid and the proportion of the cortex, the numbers of secretory cell in Beijing-Ⅲ. CONCLUSION The content of chemical composition in root tuber of Rehmannia glutinosa is higher from late October to early November, during this period, the proportion of phloem in root tuber is smallest and the proportion of xylem in root tuber is biggest, it is coincided with the traditional harvest of Rehmannia glutinosa.