鹿茸醇提物对离体脂肪组织分解的影响

Effect of Antler Ethanol Extract on Promoting the Decomposition of Adipose Tissue in Vitro

关键词:  蜡片醇提物  粉片醇提物  血片醇提物  次黄嘌呤  脂肪分解

Key words:  ethanol extract of wax slice  ethanol extract of powder slice  ethanol extract of blood slice  hypoxanthine  lipolysis

摘要:

目的 研究鹿茸醇提物对离体脂肪分解的影响及其促进脂肪分解的活性因子。方法 以梅花鹿鹿茸为原料，分别取鹿茸蜡片、粉片、血片，以75%乙醇为溶剂，提取3次后离心，合并上清液，减压回收乙醇，冷冻干燥得到鹿茸各部位的醇提物，考察鹿茸各部位醇提物对离体脂肪组织分解的作用和关系，通过高效液相色谱法测定鹿茸各部位中次黄嘌呤的含量。结果 次黄嘌呤与脂肪分解呈线性关系，推测其促进脂肪分解的活性因子可能为次黄嘌呤。次黄嘌呤、鹿茸蜡片、粉片和血片醇提物在盐酸肾上腺素存在条件下，脂肪分解率分别为472.00%，183.20%，145.56%和115.84%；无盐酸肾上腺素条件下，次黄嘌呤、鹿茸蜡片和粉片醇提物促进脂肪分解率分别为220.00%，104.74%和112.80%，血片无促进脂肪分解的作用。蜡片、粉片、血片中次黄嘌呤的含量分别为0.386，0.268，0.162 mg·g^-1。结论 蜡片促进离体脂肪分解作用最强，粉片次之，血片最弱。次黄嘌呤可能是促进脂肪分解的主要活性因子。

Abstract:

OBJECTIVE To study the effect of various parts of ethanol extract of pilose antler(AEPA) on the decomposition of adipose tissue in vitro and the active factors in decomposing of fat.METHODS Cervus Nippon antler was taken as material. Antler wax slice, powder slice and blood slice was taken and dissolved into 75% ethanol, respectively, and extracted thrice. Then the antler ethanol extract of various parts were centrifuged and combined the supernatant, last freeze-drying and studied on each part of ethanol extract to promote the role of adipose tissue breakdown in vitro. The content of hypoxanthine in each sample of velvet antler was determined by HPLC. RESULTS Hypoxanthine and lipolysis were in linear relationship, speculated the factor that activity of promoting fat decomposition might be hypoxanthine. The hypoxanthine, the ethanol extract of wax slice, powder slice and blood slice could facilitate the decomposition of rat fat regardless, when adrenalin hydrochloride was added, the decomposition rates were 472.00%, 183.20%, 145.56% and 115.84%, respectively; when adrenalin hydrochloride was not added, the decomposition rates were 220.00%, 104.74%, 112.80%, respectively, the ethanol extract of blood slice had no such effect. The contents of hypoxanthine in wax slice, power slice and blood slice were 0.386, 0.268, 0.162 mg·g^-1, respectively. CONCLUSION The wax slice promote lipolysis in vitro is the strongest, powder slice is followed and blood slice is weakest, hypoxanthine may be active factor in promoting lipolysis.