余甘子不同提取物对人消化道菌群抑菌作用的对比研究

Bacteriostastic Comparison of Different Extracts of the Fruit of Phyllanthus Emblica L. on the Microflora of the Digestive Tract

关键词:  余甘子  消化道菌群  提取物  乙酸乙酯部位  抑菌作用  最低抑菌浓度

Key words:  Phyllanthus emblica L.  gastrointestinal flora  extract  ethyl acetate portion  inhibition  minimal inhibitory concentration

摘要:

目的 通过余甘子醇提物和盐提物对消化道代表菌种大肠杆菌的抑菌试验，确定余甘子对消化道菌群的最佳抑制提取物，并确定最佳抑菌部位，从而进行该部位对消化道菌群的抑制作用研究。方法 采用管碟法用余甘子醇提物和盐水浸提物对消化道代表菌种大肠杆菌进行抑菌试验，测量抑菌圈直径及菌落生长状况来确定最佳抑菌提取物及最佳抑菌部位。采用宏量肉汤稀释法(试管法)进行该部位对消化道菌群的抑菌试验，测定最低抑菌浓度(minimal inhibitory concentration，MIC)值。结果 抑菌试验显示余甘子醇提物和盐提物均有抑菌作用，且盐提物抑菌作用较强，其最佳的抑菌部位为乙酸乙酯部位。余甘子醇提物和盐提物乙酸乙酯部位对各菌群的MIC值表明，对消化道常见菌群均有抑菌效果，且对厌氧菌的抑菌效果比需氧菌更好。结论 余甘子盐提物的抑菌效果优于醇提物，乙酸乙酯部位对消化道菌群有较强抑菌作用。

Abstract:

OBJECTIVE To investigate the bacteriostatic action of ethanol extracts and salt extracts of the fruit of Phyllanthus emblica L. on the microflora of the digestive tract, and determine its best antimicrobial part, so as to carry out its bacteriostatic effect. METHODS The bacteriositatic action of ethanol extracts and salt extracts of the fruit of Phyllanthus emblica L. was determined by cup plate method, and their MICs were measured by doubling dilution method. The bacteriostatic test of the active part to the microflora on the digestive tract was determined by macro broth dilution method. And their MICs were measured. RESULTS The test by comparing the inhibition zone diameter and colony growth conditions indicated alcohol extracts and salt extracts of Phyllanthus emblica L. had strong inhibitory effect, the best site for the antibacterial was ethyl acetate portion. The salt extracts had the better bacteriostatic action. The MICs showed not only ethanol extracts but also salt extracts had inhibitory effect, and had better effect on the anaerobe. CONCLUSION Antibacterial effect of salt extracts was better than the alcohol extract, and ethyl acetate portion had better inhibitory action on gastrointestinal microflora.