黄芪与红芪超滤物对血瘀合并短暂性脑缺血大鼠血液流变性及相关调节因子的作用

Effects of Astragali Radix Ultrafiltrate and Hedyseri Radix Ultrafiltrate on Hemorheology and Related Regulatory Factors in Rat Stasis Model Combined Transient Cerebral Ischemia

关键词:  黄芪超滤物  红芪超滤物  短暂性脑缺血  全血黏度  降钙素基因相关肽

Key words:  the ultrafiltrate of Astragali Radix  the ultrafiltrate of Hedyseri Radix  transient cerebral ischemia  blood viscosity  calcitonin gene related peptide

摘要:

目的 对比研究黄芪与红芪超滤物对血瘀合并短暂性脑缺血大鼠脑组织病理学、血液流变学及相关调节因子的作用。方法 采用注射地塞米松12 d后结扎双侧颈总动脉来复制血瘀性脑缺血大鼠模型，通过观测血液黏度、血管活性物质的含量及脑组织病理学，对比研究黄芪与红芪超滤物抗脑缺血作用及其作用机制。结果 1.32 g·kg^-1黄芪超滤物、1.68 g·kg^-1红芪超滤物可明显降低血瘀性脑缺血大鼠的全血黏度、血清一氧化氮、一氧化氮合成酶及内皮素的含量，能显著提高降钙素基因相关肽的含量，改善缺血脑组织病理学(P<0.05或0.01)。结论 黄芪与红芪超滤物具有明显的抗脑缺血作用，二者的作用未见明显差异，改善血液流变性与血流动力学相关调节因子可能是其抗脑缺血机制。

Abstract:

OBJECTIVE To comparative investigate the effect of the ultrafiltrate of Astragali Radix (RA-U) and the ultrafiltrate of Hedyseri Radix (RH-U) on hemorheology and related regulatory factors in rat stasis model combined transient cerebral ischemia. METHODS Using im. dexamethasone and ligating common carotid artery, the model of rat’s cerebral ischemia was established to evaluate the effects of the RA-U and the RH-U through detecting the levels of blood viscosity and vasoactive substance in serum. RESULTS The RA-U(1.32 g·kg^-1) and the RH-U (1.68 g·kg^-1) could degrade blood viscosity and NO, NOS and ET in serum, and upgrade calcitonin gene related peptide remarkably (P<0.05 or 0.01). CONCLUSION The RA-U and the RH-U have some protective effects on cerebral ischemia in rats, and one of the mechanisms is improving hemorheology and hemodynamics.