不同产地白薇中C21甾体皂苷的含量测定

Determination of C21-Steroidal-glycoside in Cynanchi Atrati Radix from Different Areas

关键词:  白薇  C21甾体皂苷  蔓生白薇皂苷A  含量测定

Key words:  Cynanchi Atrati Radix  C21-steroidal-glycoside  cynanversicoside A  content determination

摘要:

目的 建立白薇中蔓生白薇苷A及白薇C21甾体总皂苷的含量测定方法，并测定其在不同产地蔓生白薇中的含量。方法 以蔓生白薇苷A为指标，采用高效液相色谱法和可见分光光度法分别对蔓生白薇苷A及白薇C21甾体总皂苷进行含量测定。结果 蔓生白薇苷A和总皂苷含量分别在0.23~2.53 μg(r=0.999 7)及11.5~115 μg(r=0.999 1)内线性关系良好，平均回收率分别为99.3%(RSD=2.68%)和99.7%(RSD=2.84%)。蔓生白薇苷A的含量以河南最高，山东最低；白薇C21甾体总皂苷则以山东最高，辽宁最低。结论 此方法简便、稳定可靠，便于对白薇药材进行质量评价。

Abstract:

OBJECTIVE To establish the method for the determination of cynanversicoside A and total C21- steroidal-glycoside in Cynanchi Atrati Radix from different areas. METHODS With the content and absorbance of cynanversicoside A as index, HPLC and UV were used to determine the contents of cynanversicoside A and total C21-steroidal-glycoside in all samples. RESULTS The linear range of cynanversicoside A and steroidal-glycoside was 0.23-2.53 μg and 11.5-115 μg, respectively, with the correlation coefficient of 0.999 7 and 0.999 1; the average recoveries were 99.3% and 99.7%, with RSD of 2.68% and 2.84%. There was great difference of the samples, content of cynanversicoside A of Henan was the highest, lowest in Shandong; the total C21-steroidal-glycoside in sample from Shandong was the highest, lowest in Liaoning. CONCLUSION The method is simple, accurate and reliable for the quality control of Cynanchi Atrati Radix.