| 引用本文: | 乔洪翔,吴健,何厚洪,胡江宁,王如伟.HPLC测定银杏叶制剂中6-羟基犬尿喹啉酸的含量[J].中国现代应用药学,2014,31(10):1225-1227. |
| QIAO Hongxiang,WU Jian,HE Houhong,HU Jiangning,WANG Ruwei.Determination of 6-Hydroxykynurenic Acid in Ginkgo Preparation by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(10):1225-1227. |
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| HPLC测定银杏叶制剂中6-羟基犬尿喹啉酸的含量 |
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乔洪翔1,2, 吴健1,2, 何厚洪1,2, 胡江宁1,2, 王如伟1,2
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1.浙江康恩贝制药股份有限公司，浙江省中药制药技术重点实验室，杭州 310052;2.浙江现代中药与天然药物研究院有限公司，杭州 310052
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| 摘要: |
| 目的 建立银杏叶制剂中6-羟基犬尿喹啉酸(6-HKA)含量的测定方法。方法 色谱柱为XDB-C8(250 mm×4.6 mm，5 μm)；流动相为0.1%磷酸-乙腈-甲醇(94∶5∶1)；流速1.0 mL·min-1；检测波长为350 nm。制剂样品经粉碎后，用50%甲醇溶解，过滤后进样测定。结果 6-HKA在5.025~100.5 μg·mL-1内线性良好，峰面积与进样量呈良好线性关系，r=1.000 0；平均回收率为99.86%。6批银杏叶片剂中6-HKA的含量为0.22%~0.35%。结论 建立的方法操作简便，结果准确，重复性好。 |
| 关键词: 银杏叶制剂 6-羟基犬尿喹啉酸 含量测定 高效液相色谱法 |
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| 基金项目:国家重大新药创制项目(2011ZX09203-001-18)；浙江省重点实验室和中试基地计划项目(2009E10009) |
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| Determination of 6-Hydroxykynurenic Acid in Ginkgo Preparation by HPLC |
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QIAO Hongxiang1,2, WU Jian1,2, HE Houhong1,2, HU Jiangning1,2, WANG Ruwei1,2
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1.Zhejiang Conba Pharmaceutical Co., Ltd., Zhejiang Provincial Key Laboratory of Traditional Chinese Medicine Pharmacecutical Technology, Hangzhou 310052, China;2.Zhejiang Institute of Modern TCM and Natural Medicine Co.,Ltd, Hangzhou 310052, China
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| Abstract: |
| OBJECTIVE To establish the method of determining the 6-hydroxykynurenic acid (6-HKA) content with HPLC. METHODS XDB-C8 column(4.6 mm×250 mm, 5 μm) was used. Mobile phase was mixture of 0.1% phosphoric acid-CH3CN-CH3OH(94∶5∶1). The flow rate was 1.0 mL·min-1. The UV detector was operated at 350 nm. Tablets were comminution, dissolution with 50% methanol, and determination after filtration. RESULTS The linear range of 6-HKA was 5.025-100.5μg·mL-1(r=1.000 0); the average recovery was 99.86%. The content of 6-HKA in 6 batches of Ginkgo tablets was 0.22%-0.35%. CONCLUSION The method is simple, the result is accurate, and the reproducibility is good. |
| Key words: Ginkgo preparation 6-hydroxykynurenic acid content determination HPLC |
