HPLC同时测定裸花紫珠片中3种苯乙醇苷类成分含量

Simultaneous Determination of Forsythoside B, Acteoside and Isoacteoside in Luohua Zizhu Tablet by HPLC

关键词:  裸花紫珠片  裸花紫珠  连翘酯苷B  毛蕊花糖苷  异毛蕊花糖苷  高效液相色谱法

Key words:  Luohua Zizhu Tablet  Callicarpa nudiflora Hook. et Arn  forsythiaside B  ascoside  isoacteoside  HPLC

摘要:

目的 建立同时测定裸花紫珠片中连翘酯苷B、毛蕊花糖苷与异毛蕊花糖苷3种苯乙醇苷类成分的高效液相色谱法。方法 用50%乙醇超声提取样品，乙腈-0.4%磷酸水溶液(18.2∶81.8)为流动相，色谱柱为Agilent ZORBAX SB-C₁₈(4.6 mm×250 mm，5 μm)，柱温为30 ℃，流速为0.8 mL·min^-1，进样量为10 μL，检测波长为327 nm，检测时间为25 min。结果 连翘酯苷B、毛蕊花糖苷与异毛蕊花糖苷分别在0.936~4.680，1.852~9.260，2.140~10.700 μg内线性关系良好(r分别为0.999 1，0.999 9，0.999 9)，加样回收率分别为100.07%，99.98%，97.78%。结论 该方法简便、快速、准确，适用于裸花紫珠片等裸花紫珠各类产品中苯乙醇苷类成分的含量检测。

Abstract:

OBJECTIVE To develop an HPLC method for simultaneous determining the contents of forsythoside B, acteoside and isoacteoside in Luohua Zizhu tablet. METHODS Using 50% ethanol ultrasonic extract the sample, the chromatographic analysis was carried out on Agilent ZORBAX SB-C₁₈ column(4.6 mm×250 mm, 5 μm). The mobile phase was acetonitrile and 0.4% phosphoric acid(18.2∶81.8). The flow rate was maintained at 0.8 mL·min^-1. The detection wave length was set at 327 nm and the column temperature was controlled at 30 ℃. The sample injection volume was 10 μl. RESULTS The calibration curves were linear over the ranges of 0.936-4.680, 1.852-9.260, 2.140-10.700 μg (r=0.999 1, 0.999 9, 0.999 9) for the above compounds, respectively. The average coveries were 100.07%, 99.98%, 97.78%. CONCLUSION For this simple, rapid and accurate method is suitable for quality control and determination of products of Callicarpa nudiflora Hook. et Arn.