HPLC同时测定芍苓消银片中芍药苷、落新妇苷、绿原酸、迷迭香酸和甘草酸的含量

Simultaneous Determination of Peoniflorin, Astilbin, Chlorogenic Acid, Rosmarinic Acid and Glycyrrhizic Acid in Shaoling Xiaoyin Tablets by HPLC

关键词:  芍苓消银片  芍药苷  落新妇苷  绿原酸  迷迭香酸  甘草酸

Key words:  Shaoling Xiaoyin tablet  peoniflorin  astilbin  chlorogenic acid  rosmarinic acid  glycyrrhizic acid

摘要:

目的 建立同时测定芍苓消银片中芍药苷、落新妇苷、绿原酸、迷迭香酸和甘草酸含量的HPLC。 方法 采用Diamonsil C₁₈(250 mm×4.6 mm，5 μm)色谱柱，流动相为乙腈(A)-0.1%甲酸(B)系统，梯度洗脱；多种波长下同时检测。结果 芍药苷、落新妇苷、绿原酸、迷迭香酸和甘草酸的保留时间依次为44.2，57.5，28.6，72.3，90.6 min，回归方程依次为：y=33 605 228x-124 483，r=0.999 9，线性范围为3.25~60.5 μg·mL^-1；y=101 354 460x-100 4，r=0.999 9，线性范围为6.5~130 μg·mL^-1；y=119 599 014x+22 521，r=1.000 0，线性范围为0.98~19.6 μg·mL^-1；y=104 333 261x-365 9，r=0.999 9，线性范围为0.75~15 μg·mL^-1；y=24 700 178x+7 185，r=0.999 9，线性范围为8~160 μg·mL^-1。平均加样回收率依次为98.7%，100.4%，99.6%，102.1%，102.3%，RSD依次为为2.17%，0.74%，2.64%，1.39%，1.93%。结论 本方法操作较为简便，测定结果准确可靠，重复性好，能排除其他成分的干扰，可用于该制剂的质量控制。

Abstract:

OBJECTIVE To establish an HPLC analytical method for the determination of peoniflorin, astilbin, chlorogenic acid, rosmarinic acid and glycyrrhizic acid in Shaoling Xiaoyin tablets simultaneously. METHODS The chromatographic condition included column Diamonsil C₁₈(250 mm×4.6 mm, 5 μm), acetonitrile(A)-0.1% formic acid(B) as the mobile phase, gradient elution, the column temperature 36 ℃ and detected various wavelengths simultaneously. RESULTS The retention time of peoniflorin, astilbin, chlorogenic acid, rosmarinic acid and glycyrrhizic acid were about 44.2, 57.5, 28.6, 72.3 and 90.6 min, respectively. The regress equation for peoniflorin was y=33 605 228x-124 483, r=0.999 9 and the linear range was 3.25-60.5 μg·mL^-1; astilbin was y=101 354 460x-100 4, r=0.999 9, the linear range was 6.5-130 μg·mL^-1. Chlorogenic acid was y=119 599 014x+22 521, r=1.000 0, the linear range was 0.98-19.6 μg·mL^-1. Rosmarinic acid was y=104 333 261x-365 9, r=0.999 9, the linear was 0.75-15.0 μg·mL^-1. Glycyrrhizinic acid was y=24 700 178x+7 185, r=0.999 9, the linear was 8-160 μg·mL^-1. The average recovery of peoniflorin, astilbin, chlorogenic acid, rosmarinic acid and glycyrrhizic acid was 98.7%, 100.4%, 99.6%, 102.1%, 102.3% and RSD was 2.17%, 0.74%, 2.64%, 1.39%, 1.93%, respectively. CONCLUSION The method is specific, accurate, reproducible, and can exclude interference from other ingredients, so it can be used for quality control.