HPLC同时测定芜菁子药材中槲皮素和山奈酚含量及其指纹图谱的研究

Contents Determination of Quercetin and Kaempferol and Fingerprint Chromatograms of Brassica Rapa L. Seed by HPLC

关键词:  芜菁子  高效液相色谱法  指纹图谱  槲皮素  山奈酚

Key words:  Brassica rapa L. seed  HPLC  fingerprint  quercetin  kaempferol

摘要:

目的 建立芜菁子药材中槲皮素和山奈酚定量测定方法及芜菁子中黄酮类化合物的指纹图谱，为芜菁子质量控制提供依据。方法 采用HPLC测定芜菁子药材中槲皮素和山奈酚的含量及建立芜菁子指纹图谱，色谱条件为ODS C₁₈ (4.6 mm×250 mm，5 μm)，流动相为甲醇(A)-0.4%磷酸水溶液(B)，梯度洗脱，流速0.7 mL·min^-1，检测波长254 nm，柱温30 ℃。结果 样品中槲皮素和山奈酚的含量分别为0.013 1~0.035 6 mg·g^-1及0.022 0~0.048 0 mg·g^-1，平均回收率分别为102.8%和99.5%。芜菁子指纹图谱中有11个共有峰，对10批次芜菁子样品的指纹图谱进行相似度比较，相似度较高。结论 该方法重现性好，可行性强，可用于芜菁子的质量评价。

Abstract:

OBJECTIVE To establish a method of content determination of quercetin and kaempferol and establish the fingerprint of flavonoids ingredients in Brassica rapa L. seed, provide a basis for quality control of Brassica rapa L. seed. METHODS The contents of quercetin and kaempferol and the fingerprint of Brassica rapa L. seed were determined by HPLC with ODS C₁₈(4.6 mm×250 mm, 5 μm) and detected at 254 nm. The mobile phase was methanol(A)-0.4% phosphoric acid solution(B) with gradient elution, and the flow rate 0.7 mL·min^-1, column temperature 30 ℃. RESULTS The contents of quercetin and kaempferol were 0.013 1-0.035 6 mg·g^-1 and 0.022 0-0.048 0 mg·g^-1. The average recovery were 102.0% and 99.5%. There were eleven common peak in the fingerprint. The similarity of fingerprints of 10 batches of Brassica rapa L. seed samples were compared, and the similarity was higher. CONCLUSION The method was good reproducible. It had a strong feasibility, and can be used to control quality evaluation for Brassica rapa L. seed.