七氟烷上调糖尿病大鼠缺血再灌注肾脏Src和FAK的表达

Sevoflurane Pretreatment Upregulates Renal Src and FAK Expression in Diabetic Rats after Renal Ischemia/Reperfusion Injury

关键词:  七氟烷  糖尿病  肾脏  缺血再灌注损伤  Src  FAK

Key words:  sevoflurane  diabetes mellitus  renal  ischemia/reperfusion injury  Src  FAK

摘要:

目的 观察七氟烷预处理对糖尿病大鼠肾脏缺血再灌注(I/R)损伤肾功能及肾组织Src和FAK表达的影响。方法 将糖尿病大鼠分为假手术组、I/R组和七氟烷预处理组。给药后喂养24 h，麻醉大鼠并下腔静脉采血，测定血肌酐(creatinine，Cr)和尿素氮(blood urea nitrogen，BUN)，取肾组织使用免疫组织化学方法测定大鼠肾脏组织中Src和FAK的表达。结果 与假手术组比较，I/R组Cr、BUN明显增高，肾组织Src、FAK表达显著降低(P<0.05)；与I/R组比较，七氟烷预处理组血清Cr、BUN明显降低，肾组织Src、FAK表达显著升高(P<0.05)。结论 七氟烷预处理能改善糖尿病大鼠肾脏I/R损伤肾功能，上调Src、FAK在肾组织中的表达。

Abstract:

OBJECTIVE To investigate the effect of sevoflurane on the expression of non-receptor tyrosine kinases steroid receptor coactivator(Src) and focal adhesion kinase(FAK) in diabetic rats after renal ischemia/reperfusion(I/R) injury. METHODS The well established streptozotocin (STZ)-induced diabetes mellitus(DM) model was used. Male rats were randomly assigned to three groups: sham group, I/R group and sevoflurane pretreatment group. After 24 h, serum creatinine(Cr), blood urea nitrogen(BUN) levels, renal Src and FAK expression(immunohistochemistry) were assessed. RESULTS Compared with sham group, the Cr and BUN levels of rats in I/R group were significantly higher, while the Cr and BUN levels of rats in sevoflurane pretreatment group were significantly lower than those of rats in I/R group. The Src and FAK expression of rats in renal I/R group were significantly lower than those of rats in sham group, while the Src and FAK expression in sevoflurane pretreatment group were significantly higher than those of rats in renal I/R group. CONCLUSION Sevoflurane pretreatment in rats with diabetes mellitus protects the kidneys from I/R injury and increases renal Src and FAK expression.