HPLC同时测定豆豉片中5种有效成分含量

Simutaneously Determination of Five Active Ingredients in Black Curd Beans Tables by HPLC

关键词:  淡豆豉  高效液相色谱法  栀子苷  含量测定

Key words:  Fermented Soybean  HPLC  geniposide  content determination

摘要:

目的 建立高效液相色谱法同时测定豆豉片中栀子苷、大豆苷、染料木苷、大豆苷元、染料木素含量的方法。方法 采用Hypersil ODS 2色谱柱(200 mm×4.6 mm，5 mm)；乙腈为流动相A，甲醇为流动相B，1.8%冰醋酸溶液为流动相C，洗脱程序为0 min(A：8%，B：10%，C：82%)→28 min(A：8%，B：10%，C：82%)→28.1 min(A：0%，B：38%，C：62%)→55 min(A：0%，B：38%，C：62%)，流速为1 mL·min^-1，检测波长为254 nm，柱温：30 ℃。结果 栀子苷、大豆苷、染料木苷、大豆苷元、染料木素分别在12.36~396.00，3.50~112.00，3.36~116.00，3.00~96.00和1.63~52.00 μg·mL^-1内线性关系良好，平均回收率分别为100.0%，100.3%，99.8%，99.4%，99.9%，RSD分别为0.14%，0.97%，0.83%，0.98%，1.23%。结论 本方法灵敏度高、专属性强、重复性好，可用于本制剂的质量控制。

Abstract:

OBJECTIVE To establish an HPLC method to simutaneously determine the content of geniposide, soybean glycoside, genistin, soybean aglycone and genistein in Black Curd Beans tables. METHODS Hypersil ODS 2 chromatographic column (200 mm×4.6 mm, 5 μm) was used, the mobile phase was consisted of methyl cyanides, methanol and 1.8% glacial acetic acid as A, B and C, respectively. The elution procedure was 0 min(A: 8%, B: 10%, C: 82%)→28 min(A: 8%, B: 10%, C: 82%)→28.1 min(A: 0%, B: 38%, C: 62%)→55 min(A: 0%, B: 38%, C: 62%), the flow rate was 1 mL·min^-1, the detected wavelength was 254 nm and the temperature of column was 30 ℃. RESULTS The linear relationship of geniposide, soybean glycoside, genistin, soybean aglycone and genistein were 12.36-396.00, 3.50-112.00, 3.36-116.00, 3.00-96.00 and 1.63- 52.00 μg·mL^-1, respectively; the average recoveries were 100.0%, 100.3%, 99.8%, 99.4% and 99.9%, the RSD were 0.14%, 0.97%, 0.83%, 0.98% and 1.23%. CONCLUSION This method is sensitive, specific and reproducible, and can be used to control the quality of this preparation.