HPLC-ELSD测定红参、麦冬及其制剂中糖的含量

Determination of Sugars in Red Ginseng, Ophiopogon Japonicus and Its Preparation by HPLC-ELSD

关键词:  红参  麦冬  高效液相色谱法  蒸发光散射检测器  糖

Key words:  red ginseng  Ophiopogon japonicus  HPLC  ELSD  sugar

摘要:

目的 建立同时测定红参、麦冬及其制剂参麦注射液中果糖、葡萄糖、蔗糖、麦芽糖的含量。方法 采用Alltech Prevail Carbohydrate ES(4.6 mm×250 mm，5 μm)色谱柱，流动相为乙腈-水溶液(72∶28)，流速：1.0 mL·min^-1，柱温：25 ℃，ELSD氮气压力：3.5 bar，漂移管温度40 ℃。结果 在确定的分析条件下，果糖、葡萄糖、蔗糖、麦芽糖分别在1.01~20.20 mg，0.48~9.66 mg，0.95~19.08 mg，0.96~19.28 μg内呈良好的对数线性关系，回收率均在95%~105%内。结论 该方法简便、准确、稳定，适合用于红参、麦冬及其制剂参麦注射液中各糖的含量测定。

Abstract:

OBJECTIVE To establish a method of simultaneous determining for fructose, glucose, sucrose and maltose in red ginseng, Ophiopogon japonicus and Shengmai injection. METHODS Alltech Prevail Carbohydrate ES(4.6 mm×250 mm, 5 μm) was adopted with acetonitrile-water(72∶28) as molile phase. The flow rate was 1.0 mL·min^-1. The column temparature was maintained at 25 ℃. ELSD was was adopted with the drift temperature of 40 ℃. And Nitrogen pressure was 3.5 bar. RESULTS The linear range of fructose, glucose, sucrose and maltose were 1.01-20.20 mg, 0.48-9.66 mg, 0.95-19.08 mg and 0.96-19.28 μg, respectively. The corresponding average recovery rates were 95%-105%. CONCLUSION The method was simple, accurate and reproducible and can be used for the quality control of Red ginseng, Ophiopogon japonicus and its preparation of Shengmai Injection.