HPLC同时测定雪松松针中没食子酸、原儿茶酸和儿茶素的含量

Simultaneous Determination of Gallic Acid, Protocatechuic Acid and Catechin in Pine Needles of Cedrus Deodara by HPLC

关键词:  雪松松针  没食子酸  原儿茶酸  儿茶素  含量测定  高效液相色谱法

Key words:  pine needles of Cedrus deodara  gallic acid  protocatechuic acid  catechin  contents determination  HPLC

摘要:

目的 建立同时测定雪松松针中没食子酸、原儿茶酸和儿茶素含量的高效液相色谱法。方法 采用TC-C₁₈(250 mm× 4.6 mm，5 μm)色谱柱，流动相为甲醇(A)-0.2%磷酸水溶液(B)梯度洗脱(0~8 min，10%A，8~45 min，10%→30%A)，流速：1.0 mL·min^-1，检测波长：280 nm，柱温：25 ℃。结果 雪松松针中没食子酸、原儿茶酸和儿茶素依次在0.4~4.0，4~40和2~20 μg·mL^-1内呈良好线性关系，相关系数r分别为0.999 8，0.999 9，0.999 9，加样回收率(n=9)分别为104.7%，99.5%和105.8%。结论 该方法适用于雪松松针中没食子酸、原儿茶酸和儿茶素的含量测定，方法简单、快速、高效。

Abstract:

OBJECTIVE To develop an HPLC method for simultaneous determination of gallic acid, protocatechuic acid and catechin components in pine needles of Cedrus deodara. METHODS Agilent TC-C₁₈ column (250 mm×4.6 mm, 5 μm) was adopted. The mobile phase was methanol (A)-0.2% phosphoric acid (B) with gradient elution (0-8 min, 10%A; 8-45 min, 10%→30%A) at the flow rate of 1.0 mL·min^-1. The column temperature was 25 ℃ and the detection wavelength was 280 nm. RESULTS Calibration curves were found to be linear in the ranges of 0.4-4.0 μg·mL^-1 for gallic acid, 4-40 μg·mL^-1 for protocatechuic acid and 2-20 μg·mL^-1 for catechin. The correlation coefficients were 0.999 8, 0.999 9, 0.999 9. The average recoveries(n=9) of gallic acid, protocatechuic acid and catechin were 104.7%, 99.5% and 105.8%. CONCLUSION The method is simple, rapid and efficient, which is suitable for the determination of gallic acid, protocatechuic acid and catechin components in pine needles of Cedrus deodara.