HPLC指纹图谱结合主成分分析评价不同产地雷公藤药材质量

Evaluation of Tripterygium Wilfordii Hook. f. from Different Habitats by HPLC Fingerprint and Principal Component Analysis

关键词:  雷公藤  高效液相色谱  指纹图谱  主成分分析

Key words:  Tripterygium wilfordii Hook. f.  HPLC  chromatographic fingerprint  principal component analysis

摘要:

目的 建立雷公藤药材的HPLC指纹图谱，并结合主成分分析法(PCA)评价不同产地的雷公藤药材质量。方法 采用HPLC构建雷公藤药材的指纹图谱，并运用PCA法对指纹图谱进行统计分析，以各主要色谱峰的保留时间和峰面积为变量建立得分图和载荷图。结果 标定了雷公藤药材HPLC指纹图谱的18个共有峰，并指认了其中的6个共有峰，分别为雷公藤甲素、雷公藤内酯酮、雷公藤晋碱、雷公藤次碱、雷公藤红素、雷公藤内酯甲，11批雷公藤药材的相似度为0.587~0.913。PCA结果表明，前5个主成分的累积贡献率达到89.603%，选择这5个因子即可对雷公藤药材进行综合评价。根据综合得分可知，来自四川阿坝、四川大巴山、云南西双版纳产地的雷公藤药材质量较好。结论 HPLC指纹图谱结合PCA可以快速、客观地评价不同产地雷公藤药材的质量差异。

Abstract:

OBJECTIVE To scientifically evaluate and effectively control the quality of Tripterygium wilfordii Hook. f. (TW) by HPLC fingerprint combined with principal component analysis (PCA). METHODS An HPLC method was developed to establish the fingerprint of TW from different habitats, and PCA was applied to get score chart and loading diagram with the main components as the variables. RESULTS The common mode of the HPLC characteristic chromatographic profile was set up with 18 common peaks, and six of the common peaks were identified as triptolide, triptonide, wilforgine, wilforine, tripterine and wilforlide A. The similar degrees of eleven batches of TW to the common mode were between 0.587 and 0.913. The result of PCA showed that the accumulative contribution rate of the first five principal components was 89.603%, so as long as the five factors were chosen, the quality of TW could be evaluate basically. According to the composite score, the quality of TW from the areas of Sichuan Aba, Sichuan Dabashan, Yunnan Xishuangbanna were better than others. CONCLUSION The application of HPLC combined with PCA could quickly and objectively evaluate the quality of TW from different regions.